Thin Layer Chromatography Lab Report
Title: Thin-Layer Chromatography
Objectives:
Part1: 1. To learn the technique of TLC and the visualization of colourless components.
2. To identify an unknown drug by a TLC comparison with standard compounds.
Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf.
Introduction:
(i) General Concepts
Chromatography is a common and powerful method used to separate and analyze complex mixtures. Using this technique, the course of a reaction can be followed, and the products can be separated and isolated. In this method, the components within the mixture are distributed between two phases: a stationary phase and a mobile phase which moves through the stationary. Chromatography …show more content…
The TLC plate showed there are total four components in spinach. The Rf value of the first spot, second spot, and third spot are 0.5882, 0.9059, and 0.9059 respectively. The colours of each spot in the TLC plate have been recorded, which the compounds with Rf value of 0.5882 showed yellow colour for both visible light and UV light (254 nm) but no visible spot observed under UV light (365 nm) while the second spot showed orange colour for both visible light and UV light (254 nm) but orange-yellow spot observed under UV light (365 nm). For spot C, there was an green spot was observed under visible light and UV light (365 nm) but it showed grey spot under the short wavelength UV light. The yellow spot was predicted as the xanthophylls while the green spot is predicted as chlorophyll. Xanthophylls and chlorophylls are the pigments that usually can be found in the plant. The orange spot was predicted as the carotones and the grey spot was phaeophytins. Here is the polarity strength of compound that determine from experiment: (non-polar) Xanthophylls < Chlorophyll < Phaeophytins < Carotenes
Objectives:
Part1: 1. To learn the technique of TLC and the visualization of colourless components.
2. To identify an unknown drug by a TLC comparison with standard compounds.
Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf.
Introduction:
(i) General Concepts
Chromatography is a common and powerful method used to separate and analyze complex mixtures. Using this technique, the course of a reaction can be followed, and the products can be separated and isolated. In this method, the components within the mixture are distributed between two phases: a stationary phase and a mobile phase which moves through the stationary. Chromatography …show more content…
The TLC plate showed there are total four components in spinach. The Rf value of the first spot, second spot, and third spot are 0.5882, 0.9059, and 0.9059 respectively. The colours of each spot in the TLC plate have been recorded, which the compounds with Rf value of 0.5882 showed yellow colour for both visible light and UV light (254 nm) but no visible spot observed under UV light (365 nm) while the second spot showed orange colour for both visible light and UV light (254 nm) but orange-yellow spot observed under UV light (365 nm). For spot C, there was an green spot was observed under visible light and UV light (365 nm) but it showed grey spot under the short wavelength UV light. The yellow spot was predicted as the xanthophylls while the green spot is predicted as chlorophyll. Xanthophylls and chlorophylls are the pigments that usually can be found in the plant. The orange spot was predicted as the carotones and the grey spot was phaeophytins. Here is the polarity strength of compound that determine from experiment: (non-polar) Xanthophylls < Chlorophyll < Phaeophytins < Carotenes