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Understanding Enzymes and the Factors that Affect Them

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Understanding Enzymes and the Factors that Affect Them
RUNNING HEAD: Understanding Enzymes

Understanding Enzymes
Amanda Chung
Blanche Ely High School

Abstract The researcher conducted this experiment in order to investigate how various factors effected enzymes. Those factors were the concentration of the enzyme, the temperature it had to work in, and the pH level of the solutions as well. All three were hypothesized to speed up the rate at which the enzyme acted. A series of several tests were carried out to find the answer to each problem. First, the scientist witnessed the enzyme diastase work in a natural environment, which was a room temperature starch solution. Five trials involving enzyme and starch solution were carried out using increasing amounts of diastase to test the effect of concentration. Cold, room temperature, body temperature, and hot enzyme solutions were timed and observed to find out what effect temperature had on the reactions. To see how pH level effected enzyme reactions, the researcher used hydrochloric acid, sodium hydroxide, and distilled water to create solutions. The data recorded from the investigations were very useful in helping the scientist gain an understanding of how enzymes work and how some factors can either aid or hinder their processes.

Table of Content
Introduction……………………………………………………………………………..pg. 4
Materials and Method………………………………………………………………….pg. 10
Results….........................................................................................................................pg. 13
Discussion……………………………………………………………………………...pg. 15
Conclusion……………………………………………………………………………..pg. 19
Appendix………………………………………………………………………………pg. 20
References……………………………………………………………………………...pg. 00
Introduction
This investigation was carried out in order for the researcher to gain an understanding of enzymes in how they work, and the relative speed at which they carry out their processes. Before it was conducted, the scientist had very limited knowledge of the proteins, so



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