This in turn resulted in the particles moving faster, thus increasing collision frequency and the numbers of successful collisions as the particles have the required energy for activation. This also applies to why the reaction occurred at a slower rate at the 20 degree mark, due to lack of kinetic energy, however as the temperature rises above the optimum temperature which was discovered to be between 35 and 40 degrees Celsius then the enzymes can become denatured. This happens because the enzyme molecule vibrates more causing the weak hydrogen bonds holding it together to break. The substrate will not be able to bind as the shape of the active site is no longer complementary to the substrate enzyme complex and this cannot form. This is important in the experiment because if the yeast became denatured then it was not able to bind with the substrate (glucose) and the reaction would not be catalysed, preventing any CO2 from being formed. Because the temperature was kept constant throughout the results reflect this exactly, when the temperature exceeds the optimum the solution becomes denatured and the amount of co2 produced declines
This in turn resulted in the particles moving faster, thus increasing collision frequency and the numbers of successful collisions as the particles have the required energy for activation. This also applies to why the reaction occurred at a slower rate at the 20 degree mark, due to lack of kinetic energy, however as the temperature rises above the optimum temperature which was discovered to be between 35 and 40 degrees Celsius then the enzymes can become denatured. This happens because the enzyme molecule vibrates more causing the weak hydrogen bonds holding it together to break. The substrate will not be able to bind as the shape of the active site is no longer complementary to the substrate enzyme complex and this cannot form. This is important in the experiment because if the yeast became denatured then it was not able to bind with the substrate (glucose) and the reaction would not be catalysed, preventing any CO2 from being formed. Because the temperature was kept constant throughout the results reflect this exactly, when the temperature exceeds the optimum the solution becomes denatured and the amount of co2 produced declines