Yeast Suspension
DCP & CE
Title
The effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope.
Aim
To investigate the effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope.
Research Question
Do the different dilutions of yeast cell suspension affect the number of yeast cells per cm3 that counted using haemocytometer under the microscope?
Introduction
“The typical yeast cell is approximately equal in size to a human blood cell”. It is so small and present in such huge number that it is hard to be counted. To verify accurately the number of yeast cells, a haemocytometer need to be used as it is the most suitable for counting microorganisms under the microscope. Haemocytometer has a grid that is etched into the glass. The grid is an arrangement of squares of different sizes that allows for an easy counting of cells. It is also carefully crafted so that the area bounded by the lines is known, and the depth of the chamber is also known. Thus, it is possible to determine the number of cells in a specified volume of fluid.
Hypothesis
As yeast cell suspension become more diluted, the number of yeast cells per cm3 that counted using haemocytometer under the microscope decrease. This is because when more distilled water used, the smaller the ratio of yeast suspension to distilled water become. It means that the less yeast cell suspension will occupy the dilutions per cm3 as the yeast cell suspension get more diluted because the yeast cells had been widely distributed.
Variables
Independent variable : Different dilutions of yeast cell suspension which are one-to-hundred dilution (1/100), one-to-one thousand dilution (1/1000), one-to-ten thousand dilution (1/10,000), one-to-hundred thousand dilution (1/100,000) and one-to-one million dilution (1/1,000,000).
Dependent variable :
Title
The effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope.
Aim
To investigate the effect of the different dilutions of yeast cell suspension on the number of yeast cells per cm3 that counted using haemocytometer under microscope.
Research Question
Do the different dilutions of yeast cell suspension affect the number of yeast cells per cm3 that counted using haemocytometer under the microscope?
Introduction
“The typical yeast cell is approximately equal in size to a human blood cell”. It is so small and present in such huge number that it is hard to be counted. To verify accurately the number of yeast cells, a haemocytometer need to be used as it is the most suitable for counting microorganisms under the microscope. Haemocytometer has a grid that is etched into the glass. The grid is an arrangement of squares of different sizes that allows for an easy counting of cells. It is also carefully crafted so that the area bounded by the lines is known, and the depth of the chamber is also known. Thus, it is possible to determine the number of cells in a specified volume of fluid.
Hypothesis
As yeast cell suspension become more diluted, the number of yeast cells per cm3 that counted using haemocytometer under the microscope decrease. This is because when more distilled water used, the smaller the ratio of yeast suspension to distilled water become. It means that the less yeast cell suspension will occupy the dilutions per cm3 as the yeast cell suspension get more diluted because the yeast cells had been widely distributed.
Variables
Independent variable : Different dilutions of yeast cell suspension which are one-to-hundred dilution (1/100), one-to-one thousand dilution (1/1000), one-to-ten thousand dilution (1/10,000), one-to-hundred thousand dilution (1/100,000) and one-to-one million dilution (1/1,000,000).
Dependent variable :