Zebrafish Development

Abstract:
Using genes that control development makes it possible to identify mutants that alter development. Anterior –Posterior axis formation is controlled by protein gradients in the early embryo development. Bicoid and nanos mRNAs are both maternally provided and are localized to opposite poles of the embryo (Springfield, 2016). Bicoid proteins are concentrated at the anterior end and nanos proteins concentration is highest at the posterior end of the embryo (Springfield, 2016). The combination of how much Bicoid protein and how much Nanos protein a cell receives coordinates the axis formation (Springfield, 2016). If this pattern is altered than mutation rise by causing different expected phenotypes.

Introduction:
Genes are hereditary
In this experiment zebrafish development was studied and specifically bone formation layout and how certain mutation in cartilage-bone formation would cause a mutant phenotype. The zebrafish is a small (4–5 cm), tropical, freshwater teleost fish (ray-finned), and originally identified in the Ganges River in northeastern India. Development in the zebrafish is very fast compared to other vertebrate developmental model organisms. The embryos develop quickly in a transparent egg by making it possible to observe the entire process of vertebrate development. Organogenesis in zebrafish is rapid, and most organ primordia have formed by 24 hours of development. The 24-hour zebrafish embryo has its full set of 30 pairs of somites which are segmented blocks of mesoderm that will give rise to tissues like the vertebrae, skeletal muscle, ribs, etc... The craniofacial skeleton (jaw and head) begins with the organization of cartilage tissue to pattern the future bones. Cartilage first lays out the pattern that the bones will eventually form undergoing a process called
Results:
Figure 1: Dorsal view Figure 2: Lateral view
Wild-type zebrafish, 6dpf Wild-type zebrafish, 6dpf

Figure 2: lateral view Figure 3: dorsal view
Wild-type zebrafish wild-type zebrafish alcian blue, alizarin red stain alcian blue, alizarin red stain Figure 4: dorsal view Figure 5: lateral view
K Wild-type zebrafish K mutant type zebrafish
Alcian blue, alizarin red stain alcian blue, alizarin red