assignment on HPLC
Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj.
Title :
Assignment on HPLC (High-performance /High-Pressure Liquid Chromatography)
Introduction:
HPLC is an advanced form of liquid chromatography used in separating the complex mixture of molecules encountered in chemical and biological systems, in order to recognize better the role of individual molecules by using significantly higher pressure (50–350 bar). Compounds are separated by injecting a sample mixture onto the column. The different component in the mixture pass through the column at differentiates due to differences in their partition behavior between the mobile phase and the stationary phase. The mobile phase must be degassed to eliminate the formation of air bubbles.
Types:
Partition chromatography
Adsorption, or liquid-solid
Liquid chromatography
Ion exchange chromatography
Size exclusion, or gel, chromatography
Bioaffinity chromatography
Absorption chromatography
Composition:
Solvent
Solvent Delivery System (Pump) Injector Sample Column Detectors (Diode Array) Waste Collector Recorder (Data Collection)
Mechanism of action:
HPLC column
The column is one of the most important components of the HPLC chromatograph because the separation of the sample components is achieved when those components pass through the column. The High performance liquid chromatography apparatus is made out of stainless steel tubes with a diameter of 3 to 5mm and a length ranging from 10-30cm.
Several column types:
Normal phase: Separates analytes based on their affinity for a polar stationary surface such as silica.
Reverse phase: The packing material is relatively nonpolar (C18, C8, etc.) and the solvent is polar (methanol-water etc.) with respect to the sample. Retention is the result of the interaction of the nonpolar components of the solutes and the nonpolar stationary phase.
Title :
Assignment on HPLC (High-performance /High-Pressure Liquid Chromatography)
Introduction:
HPLC is an advanced form of liquid chromatography used in separating the complex mixture of molecules encountered in chemical and biological systems, in order to recognize better the role of individual molecules by using significantly higher pressure (50–350 bar). Compounds are separated by injecting a sample mixture onto the column. The different component in the mixture pass through the column at differentiates due to differences in their partition behavior between the mobile phase and the stationary phase. The mobile phase must be degassed to eliminate the formation of air bubbles.
Types:
Partition chromatography
Adsorption, or liquid-solid
Liquid chromatography
Ion exchange chromatography
Size exclusion, or gel, chromatography
Bioaffinity chromatography
Absorption chromatography
Composition:
Solvent
Solvent Delivery System (Pump) Injector Sample Column Detectors (Diode Array) Waste Collector Recorder (Data Collection)
Mechanism of action:
HPLC column
The column is one of the most important components of the HPLC chromatograph because the separation of the sample components is achieved when those components pass through the column. The High performance liquid chromatography apparatus is made out of stainless steel tubes with a diameter of 3 to 5mm and a length ranging from 10-30cm.
Several column types:
Normal phase: Separates analytes based on their affinity for a polar stationary surface such as silica.
Reverse phase: The packing material is relatively nonpolar (C18, C8, etc.) and the solvent is polar (methanol-water etc.) with respect to the sample. Retention is the result of the interaction of the nonpolar components of the solutes and the nonpolar stationary phase.