IB HL BIO DIalysis Lab
IB HL BIOLOGY Dialysis Lab: Exploring Osmosis and Diffusion Through A Membrane
Introduction:
Dialysis is the flow of certain solutes through a semi permeable membrane. Dialysis is usually used on patients with failing kidneys to clinically purify blood in their kidneys, or other regions of their bodies. The dialysate contains waste matter that flows from the blood our of the dialysis tubing.
Question/aim of lab:
To test the solutions inside and outside the dialysis tubing with Benedict’s solution, Iodine reagent and silver nitrate solution to find out what has remained in the dialysis tubing, and which solutes have diffused through the semi permeable membrane and into the dialysate.
Hypothesis:
The sodium chloride will definitely pass through the semi permeable membrane because it is a very small molecule, compared to the large pores in dialysis tubing. Glucose and starch have bigger molecules, and therefore are less likely to pass through the dialysis tubing
Materials:
• 250- mL beaker
• 12 test tubes (varied sizes)
• 200mm dialysis tubing
• String
• 1.0% starch solution
• 10% glucose solution
• 5% sodium chloride solution
• Benedict’s reagent
• Iodine reagent o 0.1M silver nitrate solution (in a dropper bottle)
Procedure:
PREPARING DIALYSIS TUBING
1. Place dialysis tubing in a beaker of water and allow it to soak for 5 minutes
2. Take another beaker and fill it with enough water to submerged a filled dialysis tube
3. Remove dialysis tube from water, open it, and tie one end into a knot
4. Fill the tubing less that half way with 1.0% starch solution
5. Fill the tubing to within 35mm with 10% glucose solution
6. Fill the tubing within 25mm to the top with 5% sodium chloride
7. Tie the top of the tubing with the string
8. Rise off any spilled solution on the outside of the tubing
9. Place the tubing in a beaker of distilled water
10. Let dialysis tubing remain inside for 1 day
Part I: Observation of solutions
Introduction:
Dialysis is the flow of certain solutes through a semi permeable membrane. Dialysis is usually used on patients with failing kidneys to clinically purify blood in their kidneys, or other regions of their bodies. The dialysate contains waste matter that flows from the blood our of the dialysis tubing.
Question/aim of lab:
To test the solutions inside and outside the dialysis tubing with Benedict’s solution, Iodine reagent and silver nitrate solution to find out what has remained in the dialysis tubing, and which solutes have diffused through the semi permeable membrane and into the dialysate.
Hypothesis:
The sodium chloride will definitely pass through the semi permeable membrane because it is a very small molecule, compared to the large pores in dialysis tubing. Glucose and starch have bigger molecules, and therefore are less likely to pass through the dialysis tubing
Materials:
• 250- mL beaker
• 12 test tubes (varied sizes)
• 200mm dialysis tubing
• String
• 1.0% starch solution
• 10% glucose solution
• 5% sodium chloride solution
• Benedict’s reagent
• Iodine reagent o 0.1M silver nitrate solution (in a dropper bottle)
Procedure:
PREPARING DIALYSIS TUBING
1. Place dialysis tubing in a beaker of water and allow it to soak for 5 minutes
2. Take another beaker and fill it with enough water to submerged a filled dialysis tube
3. Remove dialysis tube from water, open it, and tie one end into a knot
4. Fill the tubing less that half way with 1.0% starch solution
5. Fill the tubing to within 35mm with 10% glucose solution
6. Fill the tubing within 25mm to the top with 5% sodium chloride
7. Tie the top of the tubing with the string
8. Rise off any spilled solution on the outside of the tubing
9. Place the tubing in a beaker of distilled water
10. Let dialysis tubing remain inside for 1 day
Part I: Observation of solutions