Identification of Macromolecules Essay Example
Identification of Macromolecules
Introduction The purpose of this lab is to test for the presence of macromolecules in various foods. Tests are performed to describe the basic structure of proteins, lipids, glycerides, and carbohydrates. We are to recognize the positives and negatives in these macromolecules. Also we are to identify the roles that proteins, lipids, minerals, vitamins, and carbohydrates play in the body’s construction and metabolism.
Procedure / Results
5.1 Carbohydrate Test
1. Obtain five test tubes. Label each one
2. Use a graduated cylinder to transfer 2 mL of distilled water
3. Repeat step 2 with each of the food substances.
4. Add 10 drops of Benedict’s Solution to each test tube.
5. Gently shake the contents of each test tube.
6. Place the test tubes in the boiling water bath for 3 minutes. Remove the test tubes using test tube holders.
7. A rusty brown color in response to Benedict’s Solution indicates a large amount of simple sugars.
Table 5.1 Benedicts Test | Results: Positive results color change of red/orange for Carbohydrate | contents | Initial color | Color after heating | conclusion | Distilled water | blue | blue | neg | glucose | blue | red | pos | maltose | blue | red | pos | lactose | blue | red | pos | sucrose | blue | blue | neg | starch | Cloudy blue | Cloudy blue | neg | fructose | blue | orange | pos | Lemon juice | Teal/blue-green | green | neg | Orange juice | green | Creamy orange | pos | Non-diet soda | blue | orange | pos | Diet soda | blue | blue | neg |
5.2 Carbohydrate Test
1. Obtain five test tubes. Label each one
2. Use a graduated cylinder to transfer 5 mL of distilled water
3. Repeat step 2 with each of the food substances.
4. Add 5 drops of Iodine to each test tube.
5. Gently shake the contents of each test tube.
6. Iodine causes complex carbohydrates to turn dark blue or black
Table 5.2 Lugol’s Test | Results: Positive results
Introduction The purpose of this lab is to test for the presence of macromolecules in various foods. Tests are performed to describe the basic structure of proteins, lipids, glycerides, and carbohydrates. We are to recognize the positives and negatives in these macromolecules. Also we are to identify the roles that proteins, lipids, minerals, vitamins, and carbohydrates play in the body’s construction and metabolism.
Procedure / Results
5.1 Carbohydrate Test
1. Obtain five test tubes. Label each one
2. Use a graduated cylinder to transfer 2 mL of distilled water
3. Repeat step 2 with each of the food substances.
4. Add 10 drops of Benedict’s Solution to each test tube.
5. Gently shake the contents of each test tube.
6. Place the test tubes in the boiling water bath for 3 minutes. Remove the test tubes using test tube holders.
7. A rusty brown color in response to Benedict’s Solution indicates a large amount of simple sugars.
Table 5.1 Benedicts Test | Results: Positive results color change of red/orange for Carbohydrate | contents | Initial color | Color after heating | conclusion | Distilled water | blue | blue | neg | glucose | blue | red | pos | maltose | blue | red | pos | lactose | blue | red | pos | sucrose | blue | blue | neg | starch | Cloudy blue | Cloudy blue | neg | fructose | blue | orange | pos | Lemon juice | Teal/blue-green | green | neg | Orange juice | green | Creamy orange | pos | Non-diet soda | blue | orange | pos | Diet soda | blue | blue | neg |
5.2 Carbohydrate Test
1. Obtain five test tubes. Label each one
2. Use a graduated cylinder to transfer 5 mL of distilled water
3. Repeat step 2 with each of the food substances.
4. Add 5 drops of Iodine to each test tube.
5. Gently shake the contents of each test tube.
6. Iodine causes complex carbohydrates to turn dark blue or black
Table 5.2 Lugol’s Test | Results: Positive results