Computer Molecular Models. OBJECTIVES: Using the Spartan Student v5.0.0. Software in computer‚ determine the absorption frequencies of C=O str.‚ C-H str.‚ and C-H bend in the following molecules: 1. Benzaldehyde 2. Ethyl Benzoate 3. 3-Pentanone 4. Pentanal * For given unknown Infrared spectra‚ figure out the compounds based on the absorption frequencies of C=O str.‚ C-H str.‚ and C-H bend. DATA AND RESULTS: Assignments for unknowns A-D: Spectrum letter:
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Flame Test Laboratory Report Introduction The purpose of this lab is to observe and identify metallic ions‚ using flame tests. The traditional flame test originated when Robert Bunsen invented the Bunsen burner to test two new alkali metals he and his partner had discovered. “A flame test is an analytic procedure used in chemistry to detect the presence of certain elements‚ primarily metal ions‚ based on each element’s characteristic emission spectrum” (Flame Test 1). Using this test‚ an element
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The data we collected and the calculations we performed demonstrated that the color of a given food dye depended on which light was transmitted in the visible spectrum and which light was absorbed. A quantitative measurement of the absorbance spectrum of each food dye was obtained through spectrophotometry.1 Through this method‚ we determined that the wavelength of maximum absorbance of a given food dye was directly correlated to the color of that food dye. Further‚ darker food dyes absorbed more
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protocols and understanding what its outputs mean are very important in the development of lab technique for students of cell biology. This experiment will help laboratory students gain experience in using the spectrophotometer. Basic Laws of Light Absorption - For a uniform absorbing medium (solution: solvent and solute molecules that absorb light) the proportion of light radiation passing through it is called the transmittance‚ T‚ and the proportion of light absorbed by molecules in the medium is absorbance
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Due to the fact that it would be closer to the light source‚ the light would be slightly more intense‚ therefore the rate of photosynthesis would be slightly more advanced‚ causing a lower absorption. I also believed that Sample 1 the sample wrapped in aluminum would have the highest absorbance due to a lack of
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measure the absorption spectrum of a particular coloured substance (in this case Bromophenol Blue and Methyl Orange) at varying wavelengths of light. The goal of this experiment was to measure the given amounts of a given chemical substance that was present in a given sample of Methyl Orange and in blood. We measured the optical density at wavelengths 400 to 700 in intervals of 20 mu. For Experiment 2‚ the process of the experiment focuses on the substance and records its absorption levels at different
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applicability of the Beer-Lambert Law. 3. To investigate the derivation and limitation of Beer-Lambert Law. Introduction: In optics‚ the Beer–Lambert law‚ also known as Beer ’s law‚ the Lambert–Beer law‚ or the Beer–Lambert–Bouguer law relates the absorption of light to the properties of the material through which the light is traveling. The general Beer-Lambert law is usually written as: A = a() * b * c where A is the measured absorbance‚ a() is a wavelength-dependent absorptivity coefficient‚ b
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De Leon‚ Jo-Anna Maria N. 3ChEA Group No. 2 Experiment No. 5 Spectrophotometric Determination of pKa I. Introduction The equilibrium constant of the dissociation of an indicator in water will be determined experimentally using spectroscopic method.This experiment aims to demonstrate the principle of equilibrium and the interaction of matter with light. II. Methodology Figure . UV-VIS Spectrophotometer 10 solutions covering the entire pH range from 1 to 13 were prepared
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Spectroscopy of Food Dyes Foods and drinks are identified by two major concepts: shape and color. When individuals want to buy fruit‚ shape and color help determine ripeness and the quantity. These qualities help people spend money efficiently by buying the best fruit‚ or any food for that matter. All foods have preconceived looks‚ and if a food were to not be the same color or a different shape‚ an abnormality is noticed. The Lab Manual provides information that there are nine different FDA approved
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Crystal Field Theory Objective: To study the effect of ligands on crystal field splitting energy ( E) Theory The color of coordination compounds of the transition elements is one of their characteristic properties. These colors are due to the absorption and subsequent emission of light in the visible part of the spectrum. Light in this region of the spectrum caused promotion of d–electrons from a lower to a higher energy level. The spectra which result are gen erally referred to as Electronic
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