The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Purpose: To observe epithelial tissue by using a microscope to compare prepared slides. Introduction: Epithelial tissue covers the whole surface of the body. It is made up of cells closely packed and ranged in one or more layers. This tissue is specialized to form the covering or lining of all internal and external body surfaces. Epithelial tissue can be divided into two groups depending on the number of layers of which it is composes. Epithelial tissue which is only one cell thick is known as
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1. OBJECTIVES (a) To determine the equivalent circuit parameters of a 3-phase squirrel-cage induction motor from laboratory test data. (b) To determine the performance characteristics of an induction motor under load conditions. (c) To control the no-load speed of an induction motor. 2. INTRODUCTION 2.1 Three-phase squirrel-cage induction motors An induction motor is a transformer with a rotating secondary winding. It has two essential components: an outer stationary stator and an
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EXPERIMENT 1: TITLE: ISOMERISM AND KINETICS IN COORDINATION CHEMISTRY OBJECTIVE: 1. To prepare and study the kinetics of the interconversion of some of the isomers of a typical coordination compound. INTRODUCTION: Isomerism played a central role to establish the basic concept in coordination chemistry while reaction kinetics of coordination compound is known as the area of continuing intense research activity. The mechanism of the reactions of coordination compounds relevance
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tape them to two stools 16 cm apart‚ dropping pennies into a cup tied around the noodle? Expiremental Variables - We will be able to identify the mass of a penny and how it can test the strength of a pasta noodle as a result of the downward pull of gravity on the penny. Test Variable: The type of noodle is what I’d be changing throughout the process Outcome Variable: I would be observing how many pennies each noodle could hold counting every single one that drops into the cup until the noodle *snaps*
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Separation of the Components of a Mixture General Chemistry 1 (Chem 101)‚ ISP SCUHS Report 2 January 26‚ 2014 Abstract The analyses of mixture were to distinguish and identify homogeneous mixture by using the techniques of decantation and sublimation. By performing these techniques‚ we examined our solutions such as SiO2 (sand)‚ NH4Cl (ammonium chloride)‚ and NaCl (sodium chloride) and mixed H2O (water) with each solution after being heated. After examining our solutions‚ we made calculations
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in red blood cells) is an example of porphyrin. In this experiment‚ chlorophyll and ß-carotene were isolated using the technique of column chromatography. Column chromatography is a method used to separate and purify components in a mixture. In gravity column chromatography (used in this experiment)‚ a vertical glass column is packed with a polar absorbent and a solvent. Then the sample is allowed to pass through the column‚ which separates the different components. In this experiment‚ pigment
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Introduction In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role‚ if any‚ that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose‚ lactose will separate into galactose and glucose‚ as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions‚ and
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Limiting Reagent and Percent Yield Aim To determine the limiting reagent between the reaction of lead (II) nitrate and potassium iodide. To determine the percent yield of lead (II) iodide. Date Started: 13/4/12. Finished: 19/4/12. Data collection and processing Measurements: * Amount of distilled water: 75.0ml ± 0.5ml. * Mass of watch glass: 31.65g ± 0.01g. * Mass of watch glass + potassium iodide: 32.45g ± 0.01g. * Mass of potassium iodide: 0.8g ± 0.02g. * Mass of watch
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TABLE OF CONTENT NO. | CONTENT | PAGE | 1. | Title | 2 | 2. | Theory | 2 | 3. | Introduction | 2 | 4. | Objective | 3 | 5. | Apparatus | 3 | 6. | Procedure | 4 | 7. | Result | 6 | 8. | Calculation | 10 | 9. | Discussion | 13 | 10. | Conclusion | 14 | 11. | References | 14 | TITLE: H1 – Osborne Reynolds Demonstration INTRODUCTION: Osborne Reynold’s Demonstration has been designed for students experiment on the laminar‚ transition and turbulent flow. It consists of a transparent
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