2a: Infrared Spectroscopy and Analgesic Drug Identification Abstract: To be able to understand how organic soluble compounds could be isolated from non-organic components‚ a chromatographic separation process was used. The objective of the analgesic drug identification is to isolate the organic compounds/ active ingredients in an unknown drug. The identity of the drug was determined through melting point‚ percent recover‚ and Rf values. The comparable data for acetaminophen from the manufacturer
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Acids and Bases are two groups of chemical compounds that generally have opposite characteristics. They have there own unique traits‚ acids taste sour‚ turn litmus red while bases taste bitter and turn litmus blue. The term pH relates to measuring the acidity or alkalinity of a solution‚ such as vinegar‚ or even a damp substance like soul. The neutral pH is 7‚ which is essentially water‚ with lower numbers indicating acidity and higher numbers indicating alkalinity. Hydronium ions (H3O+) are
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The basis of this technique is the spectrophotometric measurement of the purple color produced via the reaction of thiobarbituric acid (TBA) and MDA. Concisely‚ half a milliliter of hippocampal homogenate was mixed with 2.5 ml of trichloroacetic acid (TCA) (10% w/v) solution and the mixture was boiled in a water bath for 15 min. The samples were cooled to room temperature and the absorbance at 532 nm was measured with respect to the blank
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respectively)‚ centrifuged at 500xg for 5min. The pellets were resuspended in hypotonic solutions (0.075M KCl or 1.1% tri-sodium citrate respectively) and incubated at 37°C for 20min prior to fixation with the Carnoy’s fixative (methanol : glacial acetic acid: 3:1‚ 3X). The fixed cells were spread over cold slides; air dried‚ and then stained with 7% Giemsa stain in phosphate buffer (pH6.8). A hundred well spread metaphases per animal were analyzed for chromosome aberrations in bone-marrow cells or
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Assignments in Science Class X (Term II) 4 Carbon and its Compounds IMPORTANT NOTES 9. Unsaturated organic compounds : Organic compounds in which a double or a triple bond exists between two carbon atoms in a carbon chain‚ are called unsaturated organic compounds. 10. Hydrocarbons : Organic compounds which contain only carbon and hydrogen atoms are called hydrocarbons. 11. Straight chain hydrocarbons : Hydrocarbons‚ in which all the carbon atoms are linked to one another in a straight chain
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of acetylsalicylic acid Results and Data treatment (A) Preparation of aspirin i) Details about the reactants Reaction of the acetylation of salicylic acid is following From the balanced reaction above‚ it can be seen that the stoichiometry between salicylic acid and acetic anhydride is 1: 1. In this experiment‚ 21.7mmol of salicylic acid was used to react 6.0mL of acetic anhydride and salicylic acid was limiting reagent. The expected amount of salicylic acid used: 21.7/1000*138
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primary standard to analyze acid and base solutions Acid-base titration Lab 13G Jake Shewchuk Lab 13C Dominique Genereux Purpose 13G: 1. To prepare a standard solution of oxalic acid and use it to standardize an unknown sodium hydroxide solution. Purpose 13C: 1. To titrate a hydrochloric acid solution of “unknown” concentration with standardized 0.5M sodium hydroxide. 2. To titrate a hydrochloric acid solution of “known” concentration
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Determination of the ASA Content of Aspirin Due Date: September 16‚ 2013 Experiment#: 2b Title: Determination of the ASA Content of Aspirin Aim: To determine the Molar Concentration of NaOH and HCl acid used in their Standardization processes and to determine the acetylsalicylic acid (ASA) content in Aspirin. Materials/Apparatus: materials used are the same as that outlined in the laboratory procedure prepared by the laboratory instructor. Procedure: The procedure used is the same as
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Jacobsen’s Method of Epoxidation of an Alkene | | Abstract Various types of reactions were completed to first create and then use Jacobsen’s catalyst in the asymmetric epoxidation of an unknown alkene with bleach in the laboratory. The chiral epoxide synthesized was then characterized with GC/MS and NMR. With this information the unknown alkene was able to be identified as 4-chlorostyrene. Introduction Organisms have evolved with mechanisms that use specific enantiomers of molecules
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III.MATERIALS AND METHODS 3.1. Collection and confirmation of Vibrio parahaemolyticus isolate Vibrio parahaemolyticus isolate was obtained from Cochin University of Science and Technology (Dr. I.S. Bright Singh) and grown in 1.5% TSB with 2% NaCl. A loop of the enriched culture was streaked onto thiosulphate-citrate-bile salt sucrose agar used for the selective isolation of vibrio strains (TCBS agar ).After 18-24hr incubation at 370c the cultures giving pure green colonies were randomly selected
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