Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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Lab Report An enzyme is a protein that speeds up the rates of chemical reactions. They recognize‚ bind‚ and change specific reactants. They do not change so they can catalyze the same reaction again and again. Activation energy is the amount of energy needed in order to begin a chemical reaction. A Catalyst is a substance that increases the rate of a chemical reaction without itself undergoing any permanent chemical change. Catalysts are substances or a substance that configures another substance
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Introduction: During this unknown lab report various test were performed to differentiate microbes from each other and to compare metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan
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Investigating the effect of pH on amylase activity Aim The aim of the experiment is to determine the effects of different pH and the rate of reaction on fungal amylase and starch. Introduction The enzyme amylase is found in the human body‚ it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides‚ the breakdown of starch into sugars. Amylase is present in human saliva‚ where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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Enzyme Catalysis Maltose sugar is broken apart by maltase enzyme Substrate are molecules enclosed in the enzyme Catalase: found in every living thing Takes two molecules of hydrogen peroxide and converts it irreversibly to create oxygen gas and water 2H2O2O2+2H2O Question: What variable affects the rate of enzyme catalysis most? Variables Tested: Hydrogen Peroxide concentration‚ yeast concentration‚ heat and pH Materials: 10% glucose mixture 1.5 %‚ 3% and 6% peroxide mixture Yeast
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The Effect and Rate of the Enzyme Amylase on Starch Abstract Assessing reaction speed of the enzyme amylase can be measured by the amount of glucose and maltose produced during given time intervals. I hypothesized that‚ if the reaction time is longer‚ then the amount of amylase will be larger. Enzymes are specific in their match of substrates they will breakdown – similar to a key and its lock. Since amylase is the only enzyme that breaks down starch‚ the procedure was effective and gave clear
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Omar Shbeeb Toothpick Enzyme Lab 9/25/13 Introduction Enzymes are used in all metabolic reactions to control the rate of reactions and decrease the amount of energy necessary for the reaction to take place. They are responsible for the thousands of chemical interconversions that sustain life. Enzymes are referred to highly selective catalysts‚ meaning they speed up the rate of metabolic reactions. To react‚ they need to find a perfect match with a substrate. They converge at a place called an
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Lab Report DIFFUSION AND THE EFFECT OF AMYLASE. Aim: We will interpret and explain the activity of an enzyme added to a solution. We will analyze reactions in different pH ranges. The starch solution will appear blue when mixed with iodine. But after mixing the enzyme amylase to the starch‚ the starches are broken down into simple sugars. Now‚ when the iodine is added‚ no color change exist. Instruments and materials used: 1.) Dialysis bags x2 2.)
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