one outlier that was strange was the one paper towels. Only 14 of the 15 seeds germinated in that group. The difference that we could observation was the healthiness of the plants. IV. Conclusion Paragraph 1 Recap (Evidence): The purpose of our lab was to see if the amount paper towels would affect the germination of the seeds. My hypothesis was‚ if I increase the amount of paper towels‚ then the germination rate would increase too. We observed the petri dishes in class and we would add 2 ml of
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Introduction In this lab‚ the purpose was to verify Hess’s Law. Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The enthalpy of reaction‚ ΔHrxn is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant pressure in the reaction. The change represents the difference in enthalpy of the products and the reactants and is independent of the steps
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are many conditions of the environment that can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes;
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According to the data from both the lab group and the class average‚ there is evidence that osmosis did occur in the bags. The largest change in mass was in the 1.0M sucrose bag the mass went from 12g initially to 14.2g‚ this gained 2.2g‚ an 18.3% change in mass for the group data over the duration of the experiment. The 0.2M bag went from 10.2g to 10.9g a 6.9% change in mass; the 0.4M bag went from 12.1g to 12.2g .83% change in mass; the 0.8M bag went from 10.9g to 12.2g and an 11.9% change in
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can survive in extreme environments. Halobacteria are also useful by being a good organism to perform DNA transcription‚ translation‚ and transformation on (Kramer‚ 2006). There are two different types of Halobacteria that are being observed in this lab. The first is NRC-1‚ which is also called the wild type strain. Although the pigmentation of the Halobacteria is caused by the production of the membrane protein‚ bacteriorhodopsin‚ which is a red‚ the wild type strain is pink in color. This pink color
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Meiosis and Genetic Diversity in Sordaria 979554296 Biology 110 Lab Introduction: In Israel there exists multiple spots in the mountains called Evolution Canyons‚ which are all located between a southern facing slope (SFS) and a northern facing slope (NFS). What’s particularly interesting about these locations is that despite the two slopes being on opposite sides of a small canyon‚ they exhibit extremely contrasting conditions. The SFS receives multiple times the UV radiation from the sun
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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These flies were observed in the lab by anaesthetizing them with triethylamine so they won’t fly away. They were then inserted in a vial with a foam stopper so then it was easier to observe them. A dissecting microscope was used to identify the color of their bodies‚ wing shape‚ sex characteristics
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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