Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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TITLE OF EXPERIMENT 2 : DILUTION 2.1: OBJECTIVES Upon completion of this experiment‚ students should be able to: 1. determine the concentration of coloured solution such as FeCl3 by using dilution and colour differentiating (colorimetric) techniques. 2.2: INTRODUCTION Concentration can be expressed in many different ways such as percentage volume and percentage weight etc. In laboratory‚ normally concentrations were expressed as molarity and normality. Molarity is the
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Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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Lauren Sullivan Plants Imperfections Abstract The purpose of this experiment was to plant different crosses and observe the different phenotypic ratios the plants present. The procedure of this experiment was plant six different crosses and water them correctly so that we could observe the different phenotypes and compare them to Mendel’s proposed ratios. Mendel‚ who had studied peas‚ did a similar experiment and came up with specific ratios that a monohybrid and dihybrid cross should show. His
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the pi-bonding molecular orbitals: 1 = + 2 2 = + 1.41 (two states) 3 = (two states) 4 = - 1.41 (two states) 5 = - 2 Give the electron configuration for the pi-bonding for the ground state of C8H8. c) What is value for S (total spin quantum number) for the ground state of C8H8. d) What is E‚ the pi-bonding energy‚ for the ground state of C8H8. e) Ed‚ the dlocalization energy‚ is defined as Ed = E - N ( + ) where E is the pi-bonding energy‚ N is the number
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The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
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2/28/2013 Introduction: Hess ’s law is a terminology in chemistry named after Germain Hess‚ a Swiss-born Russian chemist and physician who published it in 1840. The law states that the total enthalpy change during the complete course of a reaction is the same whether the reaction is made in one step or in several steps. Enthalpy cannot be directly measured‚ but rather the change in enthalpy. Enthalpy is described as the change in kinetic and thermal energy. Hess’s law states that the enthalpy change
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Information The purpose of the “Determining Solution ‘Concentration’ Using A Spectrophotometer” lab was to use a spectrophotometer to find the relationship of concentration and absorbance obeying the Beer-Lambert law‚ which states concentration and absorbance are directly related‚ to then further determine the concentration of three unknown solutions. With the assumption that the solutions obey the Beer-Lambert law it is predicted that as concentration increases‚ absorbance will increase as well. A spectrophotometer
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