Lab Report Enzymes (must be typed) Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL What is the Hypothesis of this experiment? (3 points) -The highest temperature will affect the reaction rate of enzymes. What is the dependent variable? (1 point) -Change of rate/product/color/absorbency. What is the independent variable? (1 pt) -The temperature. Explain in detail the procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times)
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Virtual Lab: Enzyme Controlled Reactions Worksheet 1. Which of the following does NOT apply to an enzyme: Inorganic 2. When an enzyme catalyzes a reaction: Substrate(s) bind in the active site 3. Which of the following would interfere most with the ability of an enzyme to catalyze a reaction? Reduced concentration of substrate available 4. Feedback mechanisms regulate the rate of enzyme activity‚ effectively “turning off” an enzyme in a reversible
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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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1. A) I would expect the active site of nylonase to consist mainly of polar amino acids with a few nonpolar amino acids as well because the substrate for nylonase is polar overall‚ but has many nonpolar bonds. What makes me think that the nylonase enzyme is polar is that the substrate that would bind to the active site of nylonase has extreme polarity between carbon and oxygen‚ and between hydrogen and nitrogen due to their differences in electronegativity’s‚ but it still has the nonpolar bonds between
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Effects of pH on the Function of Enzymes Rena Mototani Glen Rock High School Advanced Biology 2014-2015 Effects of pH on the Function of Catalase Rena Mototani Problem: How does the pH of a cell affect the function of the enzyme catalase? Introduction In this lab‚ we experimented the effects of pH on the function of the enzyme catalase. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia Britannica). The
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The experimental results were different from the hypothesis because while the enzyme appeared to not work as well‚ I expected a more significant change. Most of the time‚ there was only a millimeter of difference of the foam between the two samples while I expected a greater difference such as 10 millimeters. Enzymes speed up chemical reactions and at the active site‚ a substrate can be broken down or two substrates can form a larger molecule. Hydrogen peroxide is broken down by peroxidase into water
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Enzyme Lab Report Introduction The objective of this experiment was to determine if changes in pH or temperature affected the activity of enzymes‚ specifically the enzyme sucrase. Enzymes are protein molecules that act as biological catalysts to increase the speed of the reaction or to lower the activation energy of that reaction. However‚ the activity of an enzyme can be affected by physical factors such as pH and temperature because these factors alter the structure of the enzyme (Freeman‚ 2011)
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Ah Seung Chong Molecular Biology CTW: Enzyme Kinetic Dr. Cruz 07/22/2010 Enzyme kinetics Introduction Enzymes are biological catalysts or assistants‚ without enzyme many of important processes of life could not happen. Most of enzymes are proteins that help speed up chemical reactions by lowering amount of activation energy needed for the reaction1. Enzymes are usually highly selective‚ only bind to specific substrate and convert it to product at a particular rate1. The rate of the reaction
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relationship between substrate concentration and initial reaction rate provided that substrate concentration is much greater than enzyme concentration. Enzymes are essential to life as they are required for many vital metabolic reactions to occur. To adequately explain the properties of enzymes‚ it is assumed that an enzyme-controlled reaction takes place through an enzyme-substrate complex by the lock and key mechanism. It is hypothesized that a greater concentration of product is achieved through
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Enzyme Lab Report Introduction: Enzymes are proteins that enable chemical reactions. In the enzyme lab‚ the effects of concentration‚ temperature and pH on the functionality of the enzyme catalase. The enzyme lab was also about measuring reactions by capturing the oxygen that was generated by the reaction. Materials and Methods: Experiment 1‚ 2‚ & 3 Experiment 1 examined the effects of concentration on catalase activity. Experiment 2 examined the effects of concentration in temperature
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