Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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The effect of time on enzyme reaction. Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds‚ there would be the least amount of H2O2. The enzyme activity mirrored my predictions
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Interactions BigIdea 4 investigation 13 ENZYME ACTIVITY* How do abiotic or biotic factors influence the rates of enzymatic reactions? ■■BACKGROUND Enzymes speed up chemical reactions by lowering activation energy (that is‚ the energy needed for a reaction to begin). In every chemical reaction‚ the starting materials (the substrate(s) in the case of enzymes) can take many different paths to forming products. For each path‚ there is an intermediate or transitional product between reactants and
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Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
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selection Theory vs. Hypothesis Theory Broad pattern Series of supported hypotheses Hypothesis Scientific question Can be supported through experimentation * Cell Theory Discovery of cells First major conceptual breakthrough in bio Robert Hooke (1665) Discovered cells Cork (30x Magnification) Studied springs – similar to muscles Early 1800’s : all organisms consist of cells Pattern : cells exist in nature and are the basic constituent of all biological entities But where
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Association of Teachers in Biological Sciences NATIONAL STANDARD EXAMINATION IN BIOLOGY 2011-2012 Date of Examination 27th November 2011 Time 15.00 to 17.00 Hrs INSTRUCTIONS TO CANDIDATES 01. On the answer sheet‚ fill up all the entries carefully in the space provided‚ ONLY IN BLOCK CAPITALS. Use only BLUE or BLACK BALL PEN for making entries and marking answers. Incomplete / incorrect / carelessly filled information may disqualify your candidature. 02. The question paper has 80 multiple
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AP Bio DNA‚ RNA‚ Protein synthesis Study Guide DNA Structure Nucleotide structure - sugar‚ phosphate group‚ base backbone structure - alternating sugar and phosphate group directionality (5’-3’) - DNA is anti parallel. The side that sticks out s the 5’ side bases - adenine = thymine & guanine=cytosine purines vs Pyrimidines - purines have two rings while pyrimidines have one ring Bonding - hydrogen bonds Base Pairing rules - look at bases ^^ DNA Replication Semi-conservative replication
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BIO WHAT IS BIODEGRADABLE SOAP? A biodegradable soap is a cleaning agent that can decompose naturally over time. These soaps can help reduce environmental impact when hikers‚ backpackers and campers need to bathe or clean their belongings. Depending on the formulation of soap that is purchased‚ a biodegradable soap can be used on the body‚ face‚ hair‚ hands and objects such as dishes and clothes. Non-toxic soaps can be manufactured with organic oils so as to remain environmentally friendly. Oils
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Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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Potato Enzyme Lab Enzymes are protein catalysts that speed up chemical reaction in living organisms. The purpose of this lab was to; observe the breakdown of hydrogen peroxide toxin by potato’s enzyme catalase‚ determine factors that influence how quickly the reaction takes place‚ and determine factors that influence how well enzymes function. The first test‚ we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added
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