Biology Enzyme Practical
Investigate Concentration of Substrate on Catalase enzyme
1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme, as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be, that the filter paper discs that were used, will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration would be, the less time it would take for the filter paper discs to rise to the surface.
2. Experimental design.
Independent variable – is the concentration of hydrogen peroxide, in this experiment will be used 0.1, 0.2, 0.5, 1.0, 2.0 %vol from a stock solution.
Dependent variable – time in seconds for the paper disc filter to rise to the surface.
Controlled variables – Filter paper disc size 5mm diameter, same size and shape tubes, dip and clean the paste from the filter paper.
Reliability – Repeats ( 5 repeats for each concentration ) and calculating the mean average.
Control experiment – boil the mung beans, than cool them down to denature the enzymes, then carry out the experiment, keep all variables the same.
Safety risks – by doing this experiment there might be some risks if the equipment is not used properly. The tubes or anything that with glass must not be at the edge of the table, because it can fall down, and someone can cut themselves. The person that is carrying out the experiment must wear the safety goggles. Hydrogen peroxide is not dangerous, but it is an irritant, so the person must protect their eyes. Also the lab coat must be worn to avoid any splashes, in order to protect clothes or skin.
4. Analysis of results
Trend – in the graph, that has been made from the results from the table, the line goes down, I.e. as the concentration of the hydrogen peroxide increases, the time for the filter paper disc to rise to the surface decreases.
1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme, as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be, that the filter paper discs that were used, will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration would be, the less time it would take for the filter paper discs to rise to the surface.
2. Experimental design.
Independent variable – is the concentration of hydrogen peroxide, in this experiment will be used 0.1, 0.2, 0.5, 1.0, 2.0 %vol from a stock solution.
Dependent variable – time in seconds for the paper disc filter to rise to the surface.
Controlled variables – Filter paper disc size 5mm diameter, same size and shape tubes, dip and clean the paste from the filter paper.
Reliability – Repeats ( 5 repeats for each concentration ) and calculating the mean average.
Control experiment – boil the mung beans, than cool them down to denature the enzymes, then carry out the experiment, keep all variables the same.
Safety risks – by doing this experiment there might be some risks if the equipment is not used properly. The tubes or anything that with glass must not be at the edge of the table, because it can fall down, and someone can cut themselves. The person that is carrying out the experiment must wear the safety goggles. Hydrogen peroxide is not dangerous, but it is an irritant, so the person must protect their eyes. Also the lab coat must be worn to avoid any splashes, in order to protect clothes or skin.
4. Analysis of results
Trend – in the graph, that has been made from the results from the table, the line goes down, I.e. as the concentration of the hydrogen peroxide increases, the time for the filter paper disc to rise to the surface decreases.