CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase‚ 1.03 g egg white albumin‚ and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine the protein concentration
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Title: Purification of Egg white protein Name: Michael Johnson Partner: David Logad & Nandita Date: 2nd 9th September 2004 Group: Thursday 11:30am - 3:30pm Introduction Salting Out In 1888 Hofmeister that it can be possible to dehydrate a protein by adding salt to the solution‚ salting out. When a protein in a aqueous solution it is surrounded by water‚ in fact there can be up to 0.35g of water tightly bound to 1g of protein (Simpson 2004). Also the effectiveness of the salting out
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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3. The posterior pituitary gland secretes __ _ and ___ _. 4. The adult pineal gland is a small‚ shriveled remnant of the child’s. True False 5. In the blood hydrophillic hormones are often bound to transport proteins. True False True False 6. People with Type I diabetes mellitus have normal insulin levels. True False 7. Taste cells are not neurons. True False 8. Hypoglycemic hormones are secreted in response to excessively low blood glucose levels
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4A.2 RRL 4A.2.1 Coagulation of Proteins Coagulation of protein refers to sticking together‚ like a blood clot‚ usually as a result of denaturation or coming out of solution due to abnormal ionic strength or a change of solvent. Definite characteristics of the proteins are changed when they are coagulated‚ among which is loss of solubility in water and dilute salt solutions. In some instances and under certain conditions the coagulation process may be reversible. (Campbell‚ et.al‚ 1979) 4A.2.2
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Biochemistry of Photosynthesis Overall Word Equation: Water+ Carbon Dioxide( Carbohydrates+ Oxygen ((: light + chlorophyll) 1. Light Dependent Stage/ photochemical reaction/ light reactions: Word Equation: Water ( Hydrogen ions + Oxygen ((: light + chlorophyll) Site: On the Thylakoid membrane of the chloroplast Major events: • Light absorption o Chlorophyll in the thylakoids absorbs light. The
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All living things require the input of energy to exist – this energy is used to drive the thousands of biochemical reactions that occur to allow the organism to grow‚ reproduce and sustain life. This energy comes almost always from the Sun‚ in the rst instance – energy from sunlight is captured by photosynthetic organisms (e.g. plants‚ algae‚ certain bacteria) and converted into carbohydrates. These are then broken down by a process called cellular respiration‚ to produce energy-rich molecules (e
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Student Name: Jessica Sousa Student Number: M00455072 Date: 15/11/2014 BMS2113 Molecular and Metabolic Biochemistry Module Leader: BeataBurczynska Practical Tutors: Dirk Wildeboer&BeataBurczynska Formative practical worksheet (three questions) Page limit: Cover sheet + 2 pages maximum By completing the coversheet‚ students acknowledge the following on the originality of their work: I confirm that this work is my own work; where I have rewritten someone else ’s ideas in my own words‚ I have
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Biochemistry of Muscle Contraction Fred P. Guillergan M.D.‚ FPCP Outline histology and biochemistry of muscles (Striated & smooth) – Myosin – Actin‚ Tropomyosin‚ Troponin – Accessory proteins of muscles Biochemical events in muscle contraction Calcium‚ Ca2+-binding proteins and Ca2+ channels in muscle contraction Biochemistry of Cardiac & Smooth muscle contraction Energetics of muscle contraction ☻Able to understand the normal anatomy and physiology of different types of muscles
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DENATURATION OF PROTEINS Abstract The experiment aimed to use the concept of viscosity to study the effects of different denaturants on 1% albumin extract. An Ostwald viscometer was used to measure the flow time of 5 mL of the blank and native protein. These were then denatured by adding 1 mL of denaturant and had their flow time measured. The flow time from the blank to denatured protein is increasing. The specific viscosity and reduced viscosity
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