The Effect of Temperature on the Enzyme Catalase Stephen Francis Biology 183 Abstract This experiment was performed to determine the resultant effect of temperature change on the reaction between the enzyme catalase and hydrogen peroxide. This experiment was performed by measuring and comparing the amount of oxygen bubbles produced and the absorbance of the catalase and hydrogen peroxide solution over time at room temperature‚ 2°C‚ 50°C‚ and 60°C. The overall result of this experiment proved
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LAB 4 Read over the parts of the microscope and answer the following questions: 1. What do you call the lens you look through on the microscope? Ocular 2. What is the difference between the ocular lens and the objective lens? Ocular lens is the lens you look through and objective lens is the lens that is close to the stage. 3. Where do you place the slide on the microscope? the stage under the stage clips 4. Which adjustment‚ course or fine‚ do you use when you are observing the
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ABSTRACT Enzymes are biological catalysts; they cause reactions to happen that would not normally occur due to the activation energy that would be required. They bring together substrates and cause chemical reactions that are essential for life. Without enzymes life processes‚ and life in of itself‚ would not be possible. Enzymes are also special because very little of the actual enzyme is actually used up in the reaction. In this lab two different factors‚ temperature and pH‚ were tested to see
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containing different concentration levels‚ then the water would move along its concentration gradient until each side of the membrane are equal. The water moves because the membrane is impermeable to the solute and the solute concentrations may differ on either side of the membrane. Water molecules may move in and out of the cell‚ but there is no net diffusion of water. Water will move in one direction or the other‚ and this is determined by the solute or solvents concentration levels. If the two solutions
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1 Practical report: Determination of concentration using a standard curve Introduction Spectrophotometer is an instrument that confirms the measurement of specified wavelength of light that passes through medium. The measurement of light absorbance by a solution is also done with spectrophometer. Absorption spectrum Spectrometers are mainly and widely functional in identifying the components of solutions that helps determine the components’ concentrations. Compounds are experimentally identified
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Introduction: This experiment was used to examine the hypothesis that: Osmosis is dependent on the concentrations of the substances involved. Diffusion is the passage of solute molecules from an area of high concentration to an area of low concentration (Campbell & Reece‚ 2005). An example is ammonia diffusing throughout a room. A solute is one of two components in a chemical solution. The solute is the substance dissolved in the solution. The solvent‚ the other component‚ is any liquid in
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Biology lab AIM: To test a leaf for starch APPARATUS/MATERIALS : * bunsen burner‚ tripod stand‚ wire gauze(OR 90oC electric water bath OR hot plate) * 250 cm3 beaker * boiling tube * anti- bumping granules * forceps * test tube holder * white tile * leaf to be tested (hibiscus leaves are excellent) * 90% ethanol * iodine/potassium iodide solution PROCEDURE: The video above shows the steps in Testing a Leaf for the Presence of Starch. The Procedure outlined
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Biology Lab Report (F.4) * Objective: To study the transpiration of a leafy shoot using the bubble potometer * Materials and apparatus: * Bubble potometer * Leafy shoot * Scissors * Basin with water * A fan * Plumbing tape * Method: Normal Condition: 1. The bubble potometer was filled with water. 2. The stem of a leafy shoot was cut under water. 3. The bottom of the stem was wrapped by plumbing tape. 4. The stem was fitted onto the photometer
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Temperature and enzyme activity Aim: To determine the effect of which the temperature of the enzyme has on the rate of the enzyme catalysed reaction. Hypothesis: The rate of reaction of an enzyme catalysed reaction will increase as the temperature of the enzyme approaches the optimum temperature. Surpassing the optimum temperature will result in a drop in enzyme activity. Materials: 6% hydrogen peroxide Liver suspension 10 test tubes 4 beakers Thermometers Measuring cylinders Test
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BioLab3 Lab Report 5 Enzymes Student Name: Cooper Lyon I. Enzyme Structure and Function EXERCISE 1 – Preparation of an enzyme activity standard At five minute intervals over the next fifteen minute period‚ record the color intensity of the solution of each test tube. Time (min) Tube S1 Potato Extract + Catechol Tube S2 Potato Extract + Water Tube S3 Catechol + Water 0 Shade of Yellow Clear/Milky Clear/Milky 5 Shade of Yellow Clear/Milky Clear/Milky 10 Orange Clear/Milky Clear/Milky 15
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