products. The “energy barrier” represents the activation energy of a chemical reaction. In this practical the activation energy of hydrolysis of p-nitrophenyl phosphate will be determined in the presence of the enzyme alkaline phosphatase. REAGENTS * A suitably diluted solution of alkaline phosphatase * 10 nM p-nitrophenyl phosphatase * 150 μM p-nitrophenyl phosphate * 0.1 M bicarbonate buffer pH 10.0 * 0.05 M NAOH Method a) PREPARATION OF CALIBRATION CURVE In order
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2 Elements Table 1 REAGENTS 0.1M SOLUTIONS OF GROUP 2 CATIONS (AS NITRATES) Mg2+ Ca2+ Sr2+ Ba2+ Unknown D 0.02M K2CrO4 no no Light ppt Light ppt(yellow) No 0.1M (NH4)2C2O4 no Heavy ppt(white) Heavy ppt(white) Light ppt(white) Heavy ppt(white) 0.1M Na2SO4 no no Light ppt Light ppt(white) no 0.1M NaOH Light ppt Light ppt no Light ppt(white) Light ppt(white) Part II- Qualitative Analysis of Selected Anions Table 2 REAGENTS 0.1M SOLUTIONS OF GROUP
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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out of 6 questions correctly. 1. The substrate for amylase is You correctly answered: e. starch and carbohydrate. 2. Which of the following is true of enzymes? You correctly answered: c. Their activity can be affected by temperature and pH. 3. The reagent IKI tests for the presence of You correctly answered: a. starch. 4. Which of the following is not true of controls? You correctly answered: d. A negative result with a positive control is required to validate the test 5. Which of the following is
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EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz‚ Michael Ray Dela Cruz‚ Karen Angelica Domalaon‚ Abegail Dulay‚ Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor
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are specific in their match of substrates they will breakdown – similar to a key and its lock. Since amylase is the only enzyme that breaks down starch‚ the procedure was effective and gave clear results of sugar produced. With help from the stain reagent benzoic acid – I visibly saw the production of sugar molecules. However‚ I was only able to determine which of the Tubes experimented had the most or least sugar present‚ by color intensity. By operating a spectrophotometer‚ I was able to create a
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over from other steps. Every other reagent has a higher melting point‚ so it makes sense that it would be higher if the product wasn’t pure enough. They most likely contaminated the sample during the suction filtration‚ meaning that the crystals didn’t dry long enough. When water was supposed to be added drop – wise to make the solution cloudy‚ the solution was already cloudy‚ so the problem was evident there. Percent yield : See attached sheet Limiting reagent : See attached sheet III.
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Formaldehyde as a model protein crosslinking reagent. Understanding protein-protein interactions is becoming a vital study for application in the development of new age pharmaceuticals and utilization in diagnostic models. The overall aim is to gain an insight into the function and role of individual proteins with specific interest on how they react in cells‚ (Toews et al. 2008). The biopharma industry in Ireland is becoming a booming success for the economy therefore a complete understanding of
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grams CO2 is formed? How many molecules of H2O are formed? If 6.75 moles of O2 and 3.25 moles C3H8 were combined‚ how many grams H2O will be produced? Which is the limiting reagent? If 100 grams each of O2 and C3H8 were combined‚ how many moles CO2 will be produced? Which is the excess reagent? How much excess reagent will be left unreacted? Calculate the theoretical yield‚ potassium chloride produced from the reaction of 2.56 g of K and 3.85 g Cl2. If an actual experiment produces 3.81 g
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In this practical the aim was to determine the concentration of Na2CO3 in a standard solution‚ by using Hydrochloric acid to neutralise it. This experiment involved using distilled water to dissolve the sodium carbonate until 250 ml of solution were made. I had to calculate‚ from the information provided‚ how much sodium carbonate to use in the making of the solution. Using the volume and concentration of the solution‚ I was able to calculate the moles of the solution (concentration x volume). Using
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