Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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The aim of our investigation is to find out whether the use of different sugar isomers with yeast‚ will affect the rate at which the yeast respires at. The sugars to be tested are fructose‚ galactose‚ glucose‚ lactose and sucrose. Their effects on the respiration rate of yeast to be observed through the measurement (cm3) of the displaced water‚ which will tell us how much CO2 has been respired over 2 minutes. To compare the effects an average will be calculated for each and a result will be drawn
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in Two Carbohydrate Solution | Objective: 1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions. Apparatus & Equipment’s: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath‚~37°C Water bath‚~95°C Stop watch Test tube holder Materials: Carbohydrate solution
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Title: Investigation of action of saliva and hydrochloric acid in two carbohydrate solutions. Objective: To determine the action of amylase and hydrochloric acid in two carbohydrates solutions Apparatus and equipments: 1. Boiling tube 2. Metal test tube racks 3. Beaker 4. Graduated plastic dropper 5. Water bath‚ ~37ᵒC 6. Water bath‚ ~95ᵒC Materials: 1. Carbohydrate solution A 2. Carbohydrate solution B 3. Benedict’s solution 4. 3M Hydrochloric acid 5. 3M Sodium hydroxide Procedures: 1. Two
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Low Calorie Diet Versus Low Carbohydrate‚ What is the Difference? Comm/155 05/12/13 Sandra Davis Being overweight and obese has become an increasingly alarming problem in this country. In 2007 the Center for Disease Control stated that between 1980 and 2004 obesity prevalence doubled among adults‚ and over 34 percent of all American adults were overweight or obese in 2006. Obesity is known to cause an increased risk of many diseases‚ such as heart disease‚ high blood pressure‚ diabetes‚ and
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Effect of changes in substrate concentration on the reaction rate of an enzyme IB biology Internal Assessment 3/23/12 Research Question: Effect of changes in substrate concentration amount on the reaction rate of an enzyme Introduction: In this experiment‚ the substrate is hydrogen peroxide. The purpose of this investigation is to find out the relationship between the substrate concentration and the rate of reaction. Substrates are molecules that are acted upon by enzymes. For instance
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Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions Objective : To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution Results Table1: Observation Conclusion Solution A Benedict’s test: Blue coloration turned to brick red precipitate. Reducing sugar is present in the solution A. Iodine test: The coloration remained
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Experimen ChemiCal Basis of Chemical Basis of Life – Part I Carbohydrates H a n d s - o n l a b s ‚ i n c . Ve r s i o n 42-0144-00-01 LAB REPORT PHOTOS – Include two digital photos with your lab report‚ either as separate attachments to an e-mail or paste into your document. 1. Photo #1 – Take a photo of your finished experiment in exercise 2‚ testing for sugars. (The 3 test tubes after heating‚ but while still in the pan.) Be sure to take a view that shows the colors in all the tubes ^Picture
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What affect does the concentration of sugar have on the rate of respiration of yeast? Apparatus: 1. Stopwatch with a resolution of 0.001 seconds 2. 4 test tubes 3. 200 ml of yeast in a beaker 4. 200 ml of glucose solution 5. 500 ml of limewater 6. 2 delivery tubes 7. 1 test tube rack 8. 1 water bath set to 37°C 9. 2 pipettes 10. 3 measuring cylinders with a resolution of 1ml‚ ranging from 0-30ml. 11. 200 ml of water Hypothesis: The higher the concentration
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alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature and pH for the reaction of alpha-amylase and starch was predicted to be a temperature of 50°C and a pH of 5. The optimum temperature and pH for the reaction was determined by monitoring the reaction rate of alpha-amylase at different temperatures and pH’s by means of using a spectrophotometer to measure the disappearance (in absorbance) of the substrate starch. As a result‚ the
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