the method of sublimation with the use of Methylene Chloride (CH2Cl2) and Sodium Hydroxide; both can repeatedly rinse the green (unroasted) coffee beans from the caffeine. Keywords: Caffeine‚ Methylene Chloride‚ Sodium Hydroxide‚ Anhydrous Sodium Sulfate I. Introduction Caffeine is an alkaloid of the methylxanthine family. Its chemical formula is C8H10N4O2 and its systematic name is 1‚3‚7-trimethylxanthine or 3‚7-dihydro-1‚3‚7-trimethyl-1H-purine-2‚6-dione (See Fig. 1). In terms of physical properties
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Copper (II) Sulfate Purpose: The purpose of this lab is to find out how many moles of water there are in one mole of Copper Sulfate (CuSO x HO) and the percent by mass of water in the hydrate. This will be accomplished by heating a determined mass of the hydrate to remove the water‚ and by measuring the salt left over. Introduction: This laboratory experiment will be done to find out how many moles of HO are present per one mole in the hydrate CuSO. A hydrate is a compound that has a fixed number
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Experiment II. Separation Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method
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In this lab we tested the purity of water by looking for the chemicals free chlorine‚ chloride‚ sulfate‚ nitrate‚ copper‚ total hardness‚ iron‚ hydrogen sulfide‚ lead‚ pesticides‚ pH‚ total alkalinity‚ and total chlorine inside a water sample. The chemicals that produced a 0.0 result meant that there was no trace of that chemical inside the sample. The chemicals not found were copper‚ hydrogen sulfide‚ free chlorine‚ lead‚ iron‚ pesticide‚ and total chlorine. As a class we found the results of
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CC Mrs. Stilwell is a 64-year-old female here today to follow up from an emergency room visit for a motor vehicle accident. HPI The patient tells on August 4th‚ she was driving to work‚ when she was going fairly slowly only 10 to 15 miles an hour moving forward because of a green light. Another car tried to turn in front of her‚ hitting the driver-side and front of the car. This pushed her into another car which hit the other side of the front of the car‚ essentially pushing in the entire front
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Cell Lab Report Experiments : 1. PCR . 2. Protein extraction and purification . 3. Protein concentration determination . 4. SDS-PAGE . 1. The aim of experiments : 2.1 The aim of PCR experiment is to replicate some DNA dimmers by using specific enzymes used for replication in vitro which is done in lab not by living organisms. 2.2 The aim of protein extraction and purification experiment is to extract some proteins and purify them by specific methods.
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crucible after it has been heated‚ and let cool before weighing. 3. Place the crucible and cover on the triangle with the lid slightly tipped. Heat until the crucible is glowing slightly red. Record the mass 4. Using the spatula add 3g of copper sulfate hydrate crystals to the crucible and determine the mass. 5. Place the crucible on the triangle with the lid slightly tilted. Heat gently for 2-3 minutes and heat to crucible for minutes. You will observe a color change. Cool and record mass.
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factors affecting solubility 1. Effect of Temperature For some substances to dissolve in a given solvent‚ heat is absorbed. The reaction is endothermic. In this case‚ an increase in temperature increases solubility. For some substances‚ heat is released when they dissolve in a given solvent. The reaction is called exothermic. In this case‚ an increase in temperature decreases solubility. Generally‚ an increase in temperature in the solubility of solids in liquids increases solubility. But for
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1. Iron (IIII) and copper (II) sulfate solution Fill a small test tube halfway with copper (II) sulfate solution. Add a 2.0 gram iron rod to the solution and observe the reaction. 2 Fe + 3 CuSO4 = 3 Cu + Fe2(SO4)3 The new product is iron (III) sulfate‚ it contains the Fe 3+ ion which is brown. Lead (II) nitrate and potassium iodide solutions Pour about 2.0 mL of lead (II) nitrate into the test tube. Add 5 to 10 drops of potassium iodide solution to the test tube and record your observations
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This experiment was designed in order to extract caffeine from coffee grounds. In order to do this‚ filtration and extraction techniques with solvents such as calcium carbonate‚ magnesium sulfate‚ and dichloromethane were used1. Decaffeination is something that’s used around the world‚ and there is much discussion on the different methods that can be used. In this case‚ using solvents and heat was the preferred method in order to extract the caffeine. Decaffeination is often used to in order to prevent
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