Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
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unknown compound. In this experiment‚ chromatography and titration were performed to identify the unknown amino acid. Within experimental error‚ the results were consistent with the reference literature cited in this report. Experimental Thin Layer Chromatography The amino acid standards used in this experiment were Alanine‚ Glycine‚ Serine‚ and Histidine. These standards and the unknown were separated by performing a method of chromatography. Thin layer chromatography (TLC) was performed by using a mobile
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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Density Column What is density? We will be working with many different liquids. Each of the liquids has a different‚ characteristic density. One way to think about density is how thick or heavy an object is. The liquids with lighter densities will float. The liquids with heavier densities will drop to the bottom. You may have noticed that sometimes‚ liquids mix together – this is because the chemicals dissolve in each other. During our experiment‚ the liquids will stay separate because we will
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Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
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experiment is called chromatography. The materials used consisted of – 3 pieces of chromatography paper‚ a tall clear glass‚ water‚ table salt‚ 1 tsp. measuring spoon‚ an 8 oz. measuring cup‚ pencil‚ ruler‚ 6 washable markers‚ and paper towels. The markers used in this experiment have to be water soluble therefore I used washable markers made by Crayola. The colors I chose were brown‚ teal‚ red‚ orange‚ black‚ and green. The salt solution served as the mobile phase while the chromatography paper served
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Chromatography of Food Dye Chem 180-DL3 Abstract: The goal of this experiment was to use different dyes to determine the Rf value. Chromatography is done to determine the presence of a mixture in a substance. In this experiment different dyes were used such as McCormick food coloring‚ M&M’s‚ Kool-Aid‚ and the dyes that came with the lab kit. Experiment and Observation: In this experiment a 50mL beaker was filled with luke warm water and a pinch of salt water was dissolved in it
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Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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Abstract Finding the melting point of an organic substance is a practical and efficient way for scientists to identify an unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A
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