What is the difference between a.. Discussion Questions You used recrystallization to purify the product from this reaction. Could you have used column chromatography instead? Comment on the Rf values observed in your TLC analysis when predicting if chromatography would work. Recrystallization was selected as the method of purification for the Diels-Alder product due to the similar polarity between the product and the reactant maleic anhydride. Because
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reaction‚ Gas Chromatography is used to analyze the composition of the product mixture. IR spectrometry is also carried out to confirm the product. Procedure This experiment was carried out as described in Class Pak. The heat source power was set to 3 for fractional distillation and to 2 for simple distillation. For both processes‚ aluminum foil was wrapped around the heat source and flask to trap the heat and speed up the process. Results Injection Port Temperature 200 Column Temperature
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CHAPTER ONE 1.0 INTRODUCTION 1.1 Antimycobacterial Studies An Antimycobaterial is a substance that kills or inhibits the growth and activities of disease causing Mycobacteria (e.g.‚ Mycobacterium tuberculosis‚ Mycobacterium Leprae‚ etc) that are responsible for fatal diseases such as Tuberculosis and leprosy. Antimicrobacterials can either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic) (Hannan et al.‚ 2011). Mycobacterium is a genius in the family of the Mycobacteriaceae
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by means of Anion-Exchange Chromatography. Carbohydrates are weak acids with pKa values ranging between 12 and 14. At high pH they will be either completely or partially ionised depending on their pKa value. Due to the extreme alkaline conditions only polymeric anionexchange columns are suitable for
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controller! Use 40/60 mol percent propanol/butanol (no cyclohexane or toluene are used in this lab because they are more toxic) Set up fractional distillation Set heat at highest level to start‚ then turn back down to 60-80% when vapor starts up column‚ collect first sample then adjust heat as needed Not too fast or slow‚ distillation should take about 30 minutes Write procedure during distillation not when finished! You may split page with one side procedure and one side a table for volume collected
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NOTES AND COMMENT 343 DETERMINATION OF CHLOROPHYLL AND PHEO-PIGMENTS : SPECTROPHOTOMETRIC EQUATIONS~ It has been shown that chlorophyll degradation products may at times constitute a significant fraction of the total green pigments present in seawater (Yentsch and Menzel 1963; Lorenzen 1965; Yentsch 1965). These degraded forms‚ or inactive chlorophyll‚ absorb light in the red part of the spectrum; if they are present in concentrations significant relative to chlorophyll a‚ a serious error
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of essential oil from the fruits of Solanum erianthum‚ a species extensively used in traditional medicine. The essential oil from fruits was extracted by hydrodistillation and the chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS). The yield of essential oil based on the dry weight of the plant was 0.05 % (v/w). Twenty two compounds accounting for 99.2 % of the total oil were identified. The main compounds were 9‚ 12-Octadecadienoic acid (Z
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reaction was performed under reflux which sped up the reaction by continuous vapor-condensation cycles of the solution mixture as the solution boiled. The vapors were blocked from escaping the solution by a stream of cold water running up the reflux column. Also‚ to increase the reaction rate‚ an acid catalyst: sulfuric acid‚ was added to the reactant mixture (³). The main reaction is as follows: However‚ the actual reaction does not only produce the expected ester and water
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samples collected during fractional distillation were used in gas chromatography to test the purity of the distillate. When examining the vials one was missing and two had erased labels. Based on the reading from the mystery vials and the relation to the other vials the trial they could’ve come from was approximated. Based on the overall data there was very little change in the purity of the mixture. Table 3: Gas Chromatography Results from Fractional Distillate Vial trial nd20 1
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(Enclosure No. 4 to DepEd Memorandum No. ___‚ s. 2012) Format of Research Paper Investigatory papers that were reviewed by the national SRCs in the past years were found to have inadequacies in the content particularly in the areas cited below. These rules are found in the Guidelines (http://www.societyforscience.org/isef/rulesandguidelines) and in the Student Handbook (http://www.societyforscience.org/document.doc?id=12). The description of the research plan and the sample abstracts are provided
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