consist of four main chemicals. These are Chlorophylls‚ The carotenoids‚ Anthocyanin and Betacaine. The aims of this experiment is to separate the plant pigment into different colours using solvents and column chromatography. The hypothesis of this experiment is that this plant pigment from spinach will contain the chlorophyll pigment or may contain the others too. Chlorophyll: Chlorophyll a is the main component of the plant pigments. This is used for the most important process‚ Photosynthesis
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column was clamped in a vertical potion to the stand. 8mL of eluent was added to the column and the silica gel was mixed with the remainder of the eluent. The mixture was carefully poured into the column. More eluent was added to the beaker until all the silica could be poured into the column. The eluent was drained and a 1cm layer of sand was added to the top of the silica. More eluent was added and drained until it was level with the top of the sand. Extraction of pigments 3-4 baby spinach
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Chemical Separation Introduction: The objective of this experiment was to extract the pigments from spinach leaves‚ perform Thin Layer Chromatography (TLC) on the spinach leaf extract‚ and then determine the best solvent mixture to use to separate the pigments in the extract. The pigments are located inside the chloroplast walls in the cells of the spinach leaves. In order to obtain the pigments the cell walls must be broken down thus exposing the pigment containing chloroplasts. Upon
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Fresh spinach leaves | 10g | Fresh solnostemon hybrid leaves | 10g | Chromatography solvent (9:1 petroleum ether-acetone) | 3mL | Measuring cylinder (20mL) | 1 | Strainer | 1 | Acetone (100%) | 40mL | Calcium carbonate | As much as needed | Pestle and mortar | 1 | Electronic balance | 1 | Scissors | 1 | Ruler | 1 | Measuring cylinder (15mL) | 1 | Beaker (50mL) | 1 | Table 2 shows the apparatus and materials used in this experiment METHOD Preparation of spinach pigment
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Sol Hallam English Literature AS 10 February 2010 ‘They were young…’ to ‘…now forgive’ pages 3-6 How does McEwan establish the genre of tragedy in this extract? In relation to the rest of the novel‚ how typical are the tragic features used here? When thinking of a tragic novel or play‚ you may think of the great Greek tragedies. You may think of Shakespeare’s Romeo and Juliet. You may even think of a more modern play such as Death of a Salesman or Hardy’s Novel Tess of the D’Urbervilles
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Experiment 9 Thin Layer Chromatography Experiment: 9 Title: Thin Layer Chromatography Introduction: Chemical analysis is conducted on specific analytes (specific substance of interest in a mixture)‚ however it is often found that these analytes must be separated for the chemical analysis to conduct their analysis. Chromatography is a set up of laboratory a technique that is used to separate a chemical mixture. This technique is very useful as it allows us to follow the course of
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is placed in the beaker using a measuring cylinder. 3. 1ml of Brom Thymol Blue (BTB) is added into a respective tubes using a pipette. 4. 4ml from the mixture of Glucose and BTB taken and placed in test tube then added with 0.1ml of yeast extract. 5. The mixture then transferred into cuvette and
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BIOLOGY 1010L – BOTANY Laboratory Module 8. Plant Physiology (2): Plant Pigment Paper Chromatography All organisms need energy for their metabolic processes. They also need “food” to produce that energy. Plants are autotrophs (self-feeders). Plants produce their food through a process called Photosynthesis. The food that they produce is the sugar glucose. Animals and other organisms are heterotrophs (other- feeders). They must consume other organisms (plants) in order to eventually get their
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Lab Report: Understanding Photosynthesis Gen Biology Lab Abstract: This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake‚ light consumption‚ and varying pigmentation. Introduction: Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special
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Date Submitted: January 6‚ 2014 Experiment 4: Extraction and Chromatic Separation of Plant Pigments from Tomato Paste ABSTRACT In column chromatography a mixture is dissolved in a solvent and poured over a column of solid adsorbent then eluted with the same or a different solvent. This method is often used for preparative purposes; when a relatively large amount of the mixture and the components need to be isolated.
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