BIC472 Student Name: Darshan Trivedi Partner’s Name: Manan Assignment 2 POLYMERASE CHAIN REACTION Total Marks: 12 1. (2 marks) In this experiment‚ you have amplified the D1S80 locus by PCR. Explain the advantages of using this locus to distinguish one person from another. Do you think you could use a coding gene for the same purpose? Clearly explain your answers. D1S80 locus is placed on the short arm of the chromosome 1. This locus
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a serological pipet‚ mixing them thoroughly. For Part A: Absorbance of {Fe(SCN)}2+ “Knowns” Table 1: Solution Mixtures to determine Absorbance of {Fe(SCN)}2+ “Knowns.” Test Tube | Diluted Fe3+ (mL) | Stock 0.50 M KSCN (mL) | Stock of 0.1 M HNO3 (mL) | 1 | 1.0 | 5.0 | 4.0 | 2 | 2.0 | 5.0 | 3.0 | 3 | 3.0 | 5.0 | 2.0 | 4 | 4.0 | 5.0 | 1.0 | 5 | 5.0 | 5.0 | 0.0 | For Part B: Absorbance of {Fe(SCN)}2+ “Unknowns” Test Tube | Stock 0.0025 M Fe(NO3)3 (mL) | Stock of 0.0025 M KSCN (mL)
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increases. Effect of Stimulation Frequency on Contraction 2. As the frequency of stimulation increases‚ the force of contraction: increases. Materials and Methods Measurement of Threshold Stimulus 1. Dependent Variable contraction force 2. Independent Variable stimulation voltage 3. Controlled Variables muscle fiber length; frequency of stimulation; temperature Effect of Muscle Length on Contraction 1. Dependent Variable contraction force 2. Independent Variable muscle fiber length 3. Controlled
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Virtual Chem Lab 6-2 & 6-3 Anonymous CHM/110 June 16‚ 2014 Zamir Deen Virtual Chem Lab 6-2 & 6-3 Ranking Salt Solutions by pH 6-2: Ranking Salt Solutions by pH In this assignment you will be asked to rank aqueous solutions of acids‚ bases‚ and salts in order of increasing pH. This is most easily done by first identifying the strong acids that have the lowest pH‚ the strong bases that have the highest pH‚ and the neutral solutions that have a pH near 7. The weak acids will have a pH between
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LAB REPORT FOR EXPERIMENT #2: PURIFICATION OF ACETANILIDE BY RECRYSTALLIZATION Your name TA’s name Your Partner’s name Lab Section OBSERVATIONS: A. SELECTING A RECRYSTALLIZATION SOLVENT | |Solubility Test (cold) |Solubility Test (hot) | |Water |insoluble |soluble | |pet ether |insoluble
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r Virtual Lab: Enzyme Controlled Reactions Instructions 1. Go to the following web-link in order to open the Virtual Lab: http://www.mhhe.com/biosci/genbio/virtual_labs_2K8/labs/BL_02/index.html 2. Open the Virtual Lab: Enzyme Controlled Reactions 3. The virtual lab simulation will be on the right side of the screen‚ and the “Question” column will be on the left side of the screen. 4. Click the monitor in the lab simulation to watch a video about enzyme action. 5. Click the “Information”
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1. Coagulase 2. this will differentiate Staphylococcus aureus from Staphylococcus epidermidis. 3. B. Staphylococcus aureus 4. the plasma clotted (gelled)‚ indicating a positive coagulase‚ which indicates S. aureus having followed the diagnostic scheme so far. the beta-hemolysis of this particular strain of S. aureus is not diagnostic of the species. Some strains produce the hemolysin‚ while others don’t. 5. B. Alpha hemolytic and small. (There is a greenish color of the red blood
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NETW410‚ Woody Wu 1/14/14 Lab 2: Application of the Top-Down Network Design Methodology Lab Report 1. What are the business goals? (10 points) The goals of the business are to create a new network that could support the growing number of students at the campus and to create a way for off campus students to access the network. 2. What are the business constraints? (10 points) The constraints on the company are that the budget consists of revenue from property taxes and a previously
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by a plate clutch just before slip occurs. It is evident that this must depend on the friction between the plate and the plate it presses on. Hence this experiment uses three different sizes of friction plates to test the theory and assumption. 2. THEORY Consider an upper turntable which turns due to the torque T on a concentric friction plate supported by a stationary base. The inner and outer radii of the plate are ri and ro. Take an elemental plate of radius r and width dr. The axial
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of the specialized underlying structures of these life-forms. In order for us to appreciate these special adaptation‚ we first need to know how a typical plant or an animal cell organelle behaves in different water and solute concentrations. In this lab‚ we will determine the effects of hypertonic‚ isotonic and hypotonic solutions on plant and animal cells. In general when an animals cell’s placed in hypertonic solution it shrivels; a plant cell on the other hand undergoes plasmolysis. When an animal
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