Introductory Biochemical Laboratory Worksheet 10 - DNA Melting Curve Course Code: BICH172 Course Name: Introductory Biochemical Laboratory Student Name: CHUNG YUK MAN EMILY Student ID: 10198465 Group Number: 10 Date of Experiment: 16th November 2010 Q1. State the experimental aim and describe the term “hyperchromic effect”. (2 mark) The experimental aims are to study the basic structural characteristic of DNA molecules through UV spectrum and thermal denaturation
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Abstract Cold case investigation is a growing concern due to increased numbers of unsolved cases and pop culture appeal. This paper will walk you through what a cold case investigation is‚ how one begins‚ and factors such as the use of volunteers can effect the outcome of a cold case investigation. Each year the number of unsolved cases in the U.S. goes up‚ but with limiting funding and lack of manning something has to be done to keep the numbers down. A dedicated cold case unit is the best answer
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DNA Extraction from Fruit 1. What was the purpose of adding liquid soap and salt in step #1 and how does NaCl contribute to maximum DNA extraction. The purpose of using soap was to destroy the membranes inside a kiwi cell. Soap helped with that because it dissolves the membranes easily. Salt or NaCl was used to remove proteins and carbohydrates. NaCl caused the proteins and carbohydrates to precipitate. 2. Why was it necessary to “mush” the kiwi by hand? If the step was omitted‚ what
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BIOTECHNOLOGY RECOMBINANT DNA technology 1. This is a modern biotechnological advance ‚ in which a desired gene fragment can be inserted in to a cloning vector and the resulting DNA (Recombinant DNA) can be amplified in suitable host. 2. A vector can be a plasmid‚ cosmid‚bacterophage‚retroviruses‚ animal and plant viruses or artificial chromosomes like YAC‚ BAC‚or HAC.(Yeast artificial chromosome‚ bacterial........) 3. The rec. DNA produced can be amplified or cloned in a suitable vector
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DNA replication: DNA replication is a biological process that occurs in all living organisms and copies their DNA; it is the basis for biological inheritance. The process starts when one double-stranded DNA molecule produces two identical copies of the molecule. The cell cycle (mitosis) also pertains to the DNA replication/reproduction process. The cell cycle includes interphase‚ prophase‚ metaphase‚ anaphase‚ and telophase. Each strand of the original double-stranded DNA molecule serves as template
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The Discovery of the Structure of DNA Determining the structure for DNA was one of the outstanding science achievements of the 20th century. It started in the late 1940’s‚ when scientists were aware that DNA was most likely the molecule of life‚ even though many were sceptical since it was so "simple." They also knew that DNA included different amounts of the four bases: adenine‚ thymine‚ guanine and cytosine (usually abbreviated A‚ T‚ G and C)‚ but nobody knew what the molecule might look like
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Comparison chart DNA RNA Stands for: DeoxyriboNucleicAcid RiboNucleicAcid Definition: A nucleic acid that contains the genetic instructions used in the development and functioning of all modern living organisms (scientists believe that RNA may have been the main genetic material in primitive life forms). A single-stranded chain of alternating phosphate and ribose units with the bases Adenine‚ Guanine‚ Cytosine‚ and Uracil bonded to the ribose. RNA molecules are involved in protein synthesis
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Ka Zhu The Importance of the Structure of DNA Understanding ideas at a macroscopic scale is simple. Looking at a clock‚ observing and understanding the movements of the hands over the numbered surface are‚ in essence‚ all one requires to use the device. In order for innovation to occur‚ it is imperative to understand the inner workings of the device on a microscopic scale to modulate its properties. Such is the case for many innovations in science‚ from the heat engine to penicillin‚ and is no
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Who Discovered DNA? Heather Kane The discovery of deoxyribonucleic acid‚ more commonly known as DNA‚ has been the foundation for much scientific work. This fundamental discovery was credited to James Watson and Francis Crick. Many people believe that another person‚ Rosalind Franklin‚ also played a large role in the research. How much did she contribute to the discovery? Why is her name left unrecognized? This paper will discuss her part in the search and whether her name should appear next to
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Something new was also used in this case‚ dog DNA. During the investigation‚ police learned that Taylor had been walking his dog around the time Leanne’s body was placed in the woods. Forensic examiners found dog hairs on Leanne’s body. The dog hair DNA sample was sent to a university in Texas. It produced a partial profile for a dog. However‚ this was not able to be used against John Taylor because he had killed this dog before the evidence was found. The dog had been buried‚ in Taylor’s back garden
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