Bioinformatics Leigh Ann Santana DNA Extraction Lab DNA extraction is an important process because the DNA first needs to be purified away from proteins and other cellular contaminants. Cell are needed‚ because that is where the DNA is located. Inside almost every cell in our bodies is a nucleus‚ and inside each nucleus is about two meters of DNA. The following steps are needed to purify DNA from a cheek swab. Collect cheek cells‚ Burst cells open to release DNA‚ separate DNA from proteins and debris
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DNA Worksheet Misanna Gordon SCI 230 December 02‚ 2012 Mitzie Sowell Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. A DNA molecule which is abbreviation for deoxyribonucleic acid is made up of very long chains of monomers and polymers that are called nucleotides. These two chains in particular which composes of DNA strain are then formed by the grouping of the nucleotides into the polynucleotides. The
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will finish off talking about PCR‚ and then we will discuss how it can be used. If we go back to the slide of the double stranded DNA‚ and if we take that to a high temperature‚ the two strands separate‚ you then add the primers‚ which interact with ? On the strand‚ synthesis takes place in the 5-3 direction‚ then you end up with 2 molecules identical to the DNA‚ and then you do another round‚ so it’s an exponential increase. There are different enzymes and polymerases‚ which come from bacteria
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A DNA strand contains a complete representation of everything about our physiology. It also contains instructions on how to form our body by repeated divisions of a single cell. Each cell needs to know when it should‚ split into two‚ split into different kinds of cell for tissue differentiation. Cells also need to know when to stop growing because the body or organ is mature‚ and when it needs to replace tissue lost by injury. All of that is encoded into one molecule. So in other words the benefit
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DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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This lab must be typed. Title DNA Fingerprinting Purpose Why are we doing this lab Background 1. What are restriction enzymes 2. When added to a DNA sample‚ what do restriction enzymes do 3. What do you call the specific sequence of bases the enzyme is searching for 4. What is a restriction digestion 5. What is the purpose of the water bath 6. The electrophoresis apparatus creates an electrical field with positive and negative poles at the ends of the gel. DNA molecules are negatively charged.
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DNA REPLICATION At the replication origin DNA helicase attaches to a strand of DNA and begins to break apart hydrogen bonds in order to unravel a section of the double helix. The section of DNA that is unwound is called the replication bubble and the “Y” shaped sections are called the replication forks. In order to stop the unwound section from binding back together‚ single strand binding proteins react with the single strand portions on the DNA causing them to stay separated. Although the leading
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DNA‚ RNA‚ PROTEINS STARTS WITH ? Name _______________________________ 1. DNA that is spread out in the nucleus of a non-dividing cell so it can be read is called _C_ __ __ __ __ __ __ __ __ . 2. The group of 3 nitrogen bases in the mRNA message that is read together is called a _C_ __ __ __ __. 3. In dividing cells‚ the DNA is scrunched into _C_ __ __ __ __ __ __ __ __ __ __ so it can be moved. 4. The mRNA message tells the ribosomes which _A_ __ __ __ __ _A_ __ __ __ to put in next
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Sam Rausser Wednesday‚ August 17‚ 2011 3:38 PM Once glucose is made‚ glucose molecules can be converted (by plants) to… 1) monosaccharides‚ like fructose 2) Disaccharides a. Maltose (glu‐glu) seeds b. Sucrose (glu‐fru) sugar cane‚ sugar beets 3) Polysaccharides a. Starch‚ food storage in roots (chains of glucose) b. Cellulose‚ plant structure (chains of glucose) 4) Amino Acids a. Glucose + N from ground 5) Lipids‚ specifically oils which are energy source in seeds & phospholipids found in membranes
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least 100 words: 1. Describe the structure of DNA. DNA is a structure of specific molecules and a complex mixture of chemicals. DNA is a nucleic acid‚ which is a group of complex compounds that can be found in all living cells or viruses‚ and controls cell health and function. Nucleic acids are composed of polymers and monomers‚ which are referred to as nucleotides. There are four different types of nucleotides that make up the structure of DNA‚ which are abbreviated A‚ C‚ T‚ and G. Covalent
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