SDS-PAGE is a standard technique for determining the abundance and molecular weight of a protein using an anionic detergent (sodium dodecyl sulfate or SDS) which gives the molecules a net negative charge. The charged molecules are pipetted into a gel and then
Premium DNA Gene Bacteria
have to be adopted to utilize them for the conversion into value-added products [Nand‚ (1998)]. Pectin exists in varying amounts in fruit cell walls and has important nutritional and technological properties‚ mainly because of its ability to form gels (Westerlund etal‚ 1991). Pectin is a polysaccharide having properties such as gelation and emulsion stabilization which make it useful in the manufacture of food‚ cosmetics‚ and medicine. It is a normal constituent of food and may therefore be safely
Premium
Question 1. Carla would like to open a small dress shop. At the present time‚ she believes that the chances of a successful or unsuccessful dress shop are about the same (50%). If Carla opens a shop and it is successful‚ she expects to make a profit of $100‚000. If she opens a shop that proves to be unsuccessful she will lose $50‚000. On the other hand‚ If Carla chose not to open her own shop‚ she would have the option of investing in a dress shop being started by her best friend Amanda. The probabilities
Premium Dow Jones Industrial Average Decision theory Approximation
How is Vitamin C manufactured? Ascorbic Acid Also known as: l-ascorbic acid‚ vitamin C‚ ascorbate A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets‚ and necessary to maintain connective tissue and bone. Its biologically active form‚ vitamin C‚ functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant. http://pubchem.ncbi.nlm.nih.gov/summary/summary
Premium Flavor Food preservation Vitamin C
Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
Premium Molecular biology DNA
How to Complete Your IOSH Project Assignment Silicon Beach Training Silicon Beach Training · 86 Gloucester Road‚ Brighton‚ BN1 4AP +44 (0)1273 622272 - info@siliconbeachtraining.co.uk Follow Silicon Beach Training Completing your IOSH Project Assignment PAGE 1 Introduction Part of achieving your IOSH Managing Safely accreditation involves completing a project assessment about your workplace. Here we’ve put together our IOSH Project Assignment guide to help you complete your assessment. Each
Premium Risk Risk assessment Risk management
incubating the reaction tubes in a 37°C waterbath for 45 minutes. Finally‚ after the incubation was complete‚ 5 µl of 10x gel loading solution was added to each reaction tube to stop the reaction. Once again‚ the reaction tubes were covered and tapped gently to mix the solution and stored in the refrigerator for gel electrophoresis. Table 6.1 Summary of Restriction Enzyme Digestion
Premium DNA Protein Molecular biology
amount of DNA ⎯ even from the whole genome. This specific sequence can be found through a combination of two different techniques: Agarose Gel Electrophoresis and Hybridization‚ which is known as Southern Blotting. This technique if performed in three phases: (I) prepare the gel and (II) make the blot‚ (III) hybridize and visualize blot. In phase one‚ the gel is prepared in three steps: (1) chemical digestion‚ (2) electrical separation‚ and (3) chemical denaturation. In the first step‚ the DNA sample
Premium DNA Molecular biology Water
The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
Premium DNA Genetics Gene
LASER ( Light Amplification by Stimulated Emission of Radiation). * In 1967‚ Charles Kao and George Bockham of the Standard Telecommunications Laboratory proposed the cladded fiber cables. * In 1970‚ Robert Maurer‚ Donalk Keck and Kapron of Corning Glass developed the first fiber optics with losses less than 2dB/Km. * In 1980’s‚ losses in fiber optics were reduced to as low as 0.16 dB/Km. This is due to the development of high-quality light sources and detectors. * In 1990’s‚ the photonic
Premium Optical fiber Laser