Genetic Synthesis

The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger, at roughly about 6000 base pairs and 6800 base pairs, than the predicted size of 2315 and 3119 base pairs. The fragment size of the three control candidates in wells numbered four, five, and six align with the DNA ladder at about the 6500 base pair mark, which better corresponds with the predicted size of 4969 base pairs. This could have been the result of the fragments not completely migrating to their exact position during gel electrophoresis. While the gel was running the ladder and bands were unable to spread fully across the gel as they should have for the time allowed. An additional 30 minutes were used to run the gel to see if the bands would separate better. The transition, nonsense mutation found in the
The exponential growth of this technology continues to benefit not only the science and medical fields, but also the agricultural field. Genetically engineered crops are becoming more and more prevalent for their ability to produce desired yields and products. In addition, this technology can also produce novel vaccines and protein therapies, similar to the insulin production. The implication of this technique and others involving recombinant DNA technology allows for further knowledge of cloning and genetic engineering techniques. With the ever advancing technology, the ease and precision of these genetic engineering techniques are sure to become