Abstract: This lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won’t denature in cold temperatures‚ but have slowed molecular activity. pH will also have an affect on an enzymes efficiency‚ when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable
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learning how to measure the pH of several different liquids as well as learning how to change the pH of those liquids by adding other acidic and alkaline mixtures. Materials: Graduated cylinder- used to measure the various liquids 4 100 mL beakers- used to hold liquids when measuring pH pH meter- used to measure the pH of the various liquids Tap water- liquid we are trying to find pH of Distilled water- liquid we are trying to find pH of Soda- liquid we are trying to find pH of Milk- liquid we
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Determining the Optimal Temperature and PH of Barley Amylase Abstract The purpose of this experiment was to find the optimal temperature and pH of barley alpha-amylase. I hypothesize that the optimal temperature would be 55 degrees Celsius and the optimal pH would be 5.5. In this experiment‚ the starch is used as a substrate to examine the optimum temperature and pH for the reaction of alpha amylase. It is known that the measuring of disappearance (absorbance) of the substrate starch with iodine
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compounds that generally have opposite characteristics. They have there own unique traits‚ acids taste sour‚ turn litmus red while bases taste bitter and turn litmus blue. The term pH relates to measuring the acidity or alkalinity of a solution‚ such as vinegar‚ or even a damp substance like soul. The neutral pH is 7‚ which is essentially water‚ with lower numbers indicating acidity and higher numbers indicating alkalinity. Hydronium ions (H3O+) are formed when water molecules (H2O) bond with
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September 20‚ 2010 EXPERIMENT Acids‚ Bases‚ and Neutrals Problem Are common house-hold products pHs balanced and neutral? Information pH measures how acidic or alkaline a solution is. Its concept was introduced in 1909 by biochemist Soren Sorensen. The scale ranges from 0-14. Solutions that range from 0 to 6 are considered to be Acidic. Solution that’s on the Acidic end of the scale is low in ph‚ high in hydrogen ion‚ and low in hydroxyl ions. An acid have a sour taste‚ reacts with metal‚ are
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using Catalase Introduction An enzyme is a globular protein which functions as a catalyst that speeds up the rate of a reaction without being consumed in the reaction. An enzyme-catalyzed reaction occurs when a substance called the substrate binds onto the active site of the enzyme. This joining allows for a reduction in the activation energy and the reduction in activation energy helps the reaction to occur at a quicker rate. Enzyme reaction can be affected by some factors such as pH‚ temperature
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professor! I chose to discuss topic 2 this week. The topic is Discuss how buffers help to regulate body pH. The pH level in the blood is maintained by the kidneys and the lungs. The pH level on the blood should be at 7.4. In order for the kidneys and lungs to maintain this pH level it affects the buffers in the blood. The buffer is a substance that is resistant to the change in the body’s pH level. Basically the buffers can make an acid or base less potent and try and neutralize it so the body is
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The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise‚ the study of enzyme catalase‚ we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment. Our class performed
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Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing acetic
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During this experiment the change in pH of Citric acids is measured from start to equilibrium in mL. This experiment was tested by titration it had two separate trials. Through observations it was shown that the more concentrated acids needed more drops of NaOH to reach its equilibrium then the less concentrated acids. Introduction Sodium Hydroxide (NaOH) is a strong base and when it is titrated with a strong acid the equivalence point will be expected to be a pH of 7 (wyzant.com‚ Feb. 12). NaOH
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