Laboratory 2: Scientific Methodology & Enzyme Activity Objective: The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes‚ temperature‚ and PH level). Hypothesis: Part a: If different amounts of enzyme solution are added to the hydrogen peroxide‚ then the highest amount of enzymes will have the greatest reaction rate because enzymes catalyze reactions‚ meaning more oxygen
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The Effects of Temperature on the Action of Diastase on a Starch Suspension Hypothesis: The practical being carried out is to observe the effects of temperature on starch break down using a synthesized version of salivary amylase‚ this being Diastase. The starch will be placed into the Diastase and water and then placed in baths of water of different l. temperatures. The test tube containing water will have little or no reaction at all. However‚ the test tube containing the Diastase
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Enzymes are a major role in the process of accelerating chemical reactions. Environmental factors affect the enzymes‚ These environmental factors include ph levels and body temperature. While both of these environmental factors affect the enzyme‚ body temperature affects the enzymes the most and can result in death. Enzymes are affected by high body temperature. Enzymes are highly specific and they work at certain temperature levels depending if it’s hot or cold. However‚ when the
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understand the process of fermentation of yeast in different concentrations of sucrose. The experiment worked with yeast and sugar (sucrose and glucose) to determine the rate of fermentation by testing the pressure of C02 in the test tube. The experiment tested the metabolic capability of yeast anaerobically meaning no oxygen was present (this was ensured by the thin layer of oil on the top of the solution). This means that the metabolic rate of the yeast could be determined by testing the pressure in
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Enzymes Enzymes are… * Biological catalysts Lower the energy level needed for a biochemical reaction to occur. This energy level is called activation energy. * Proteins Polypeptide chains made up of 100’s-1000’s of amino acids in a specific sequence. * Do not get “used up” in a reaction The number of “uses” of an enzyme depends on the enzyme. * Work more efficiently at certain optimum temperatures. * They are “reaction-specific”. Each enzyme is included in one reaction.
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The effect on rate of an enzyme catalysed reaction by different objectives which include effect of substrate‚ temperature‚ ph and effect of a competitive inhibitor phosphate ions. This is determined by the reaction of hydrolysis by p-nitrophenylphosphate (PNP) as a substrate by the enzyme phosphatase. Abstract The hydrolysis of p-nitrophenyl phosphate has been studied in human red blood cells. To see if hydrolysis was related to the functioning of the sodium pump. Acid phosphatase catalysis’s
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Abstract This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to
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Brooke Strowd AP Biology Ms. Butler 28 January 2013 I. Title: Effect of a Catalyst on Reaction Rate II. Introduction: The basis of the experiment is the enzyme; an enzyme is a biological molecule that acts as a highly selective catalyst. By combining with a substrate‚ an enzyme is able to create a new product that helps the body function. (Ex. Lactose/Lactase) A substrate is a molecule an enzyme acts upon‚ the two combine at an area called an active site. This active site allows induced fit
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Compare the Rate of Carbon Dioxide Production by Yeast under Anaerobic Conditions using different Carbohydrate Substrates. Hypothesis. The hypothesis that I draw is that "" out the five carbohydrate substrates that I will use‚ Glucose will produce the highest volume of Carbon Dioxide at every five-minute interval. Null Hypothesis. The null hypothesis that I am composing is that "" the five carbohydrate substrates that I am to use will not produce any Carbon Dioxide. Scientific Research. Under
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How does pH affect the activity of the Amylase? Abstract: In my experiment I aimed to observe how ranging pH levels will affect the rate in which amylase will break down the starch molecules. I will be measuring the time it takes for the dark liquid to disappear and leave a yellow brown liquid to be shown‚ which would show that there is no starch present in the solution because it would have broken into maltose by adding amylase. Results did not fully demonstrate what we expected in our hypothesis
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