a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH nullifies the negative charge on the protein
Premium Acid Acid dissociation constant Base
Carboxylic acids are compounds which contain a carboxyl group‚ -COOH group‚ where the -COOH group is attached either to a hydrogen atom or to an alkyl group. One of the example of carboxylic acid is benzoic acid( benzene carboxylic acid) which has a benzene connected to the carboxyl carboxyl group‚ -COOH group. It has a formula of C6H5COOH. The carbon atom of a carboxyl group has a high oxidation state. Therefore‚ that many of the chemical reactions used for their preparation are oxidations. In
Premium Oxygen Alcohol Hydrogen
The purpose of this experiment was to synthesize triphenylmethanol from a Grignard reagent. The Grignard reaction technique was used in this synthesis but due to the fact that it is such a strong nucleophile and base‚ it was important to prevent water from interfering with the Grignard reaction. Purity of the product was determined by measuring the melting point. Reagent Table: Structure Name Molecular formula Molar mass Density Melting point Boiling Point Diethyl ether C4H10O
Premium Magnesium Oxygen Diethyl ether
tested a sodium chloride solution. Along with the tested solution‚ control groups (water and sodium phosphate) were used to be help understand whether or not NaCl was a buffer. Water was the negative control group and sodium phosphate was the positive control group. If NaCl was a buffer than the pH would be stabled as the sodium phosphate buffer. If NaCl was not a buffer than the pH would fluctuate like the negative control‚ water. During the first trial and prior to the drops of 0.5 M of HCl acid‚ the
Premium PH Acid Hydrochloric acid
Classifying Chemical Reactions Purpose: The purpose of this experiment is to observe a variety of chemical reactions and to identify patterns in the conversion of reactants into products. Apparatus: • Bunsen or lab burner -Test tube clamp • Butane safety lighter - Test tube rack • Evaporating dish - Wash bottle • Forceps or crucible tongs - Wood Splints • Heat resistant pad • Litmus paper • Pipets • Spatula • Test tubes Materials: Ammonium carbonate‚
Premium Chemical reaction Sodium hydroxide Chlorine
Measuring the enthalpy change of vaporisation of water You should attempt this part of the activity even if you have not seen the demonstration of the practical. 1. Calculate a value for ∆Hvap for water in KJ mol-1 using the data from the demonstration. Also‚ calculate ∆Hvap from the following data gathered by a student; 8100 J were needed to distil 2.95g of water. a) Explain why the boiling tube was surrounded with expanded polystyrene (or other similar insulation)‚ but the condenser was left
Premium Water Boiling Distillation
Introduction – Lab Report What is a Chemical Reaction? A chemical reaction is a change in matter that produces one or more new substances. A chemical change or reaction occurs when bonds are broken and new ones are formed. The formation and dissolution of these bonds are dependent upon environment changes. Even without the usage of microscopes‚ chemical reactions are usually apparent to the naked eye. The two main kinds of changes that one can observe are the formation of new substances and changes in energy
Premium Chemistry Chemical reaction Temperature
Acids‚ Bases and Buffers Lab Acids‚ Bases and Buffers Lab Results: The experimental results for part one is as follows: Part One Data Table | Initial pH | Final pH | Test Tube A | 6 | 1 | Test Tube B | 4 | 4 | Test Tube C | 4 | ----- | Test Tube D | 4 | 4 | Test Tube E | 6 | 11 | The experimental results for part two is as follows: Part Two Data Table | Before CO2 was Added | After CO2 was Added | Colour | Blue/green | Light green/yellow | pH Level | 8.0pH | 5.0pH |
Free PH
rust)]. The thermite reaction is a redox reaction‚ where Aluminum reduces the oxide of another metal. For example‚ when using iron oxide (as I did) the equation would be Fe2O3(s) + 2 Al(s) -> Al2O3(s) + 2 Fe(l). Black or blue iron oxide (Fe3O4) could also be used. Other examples of possible oxides are manganese thermite (MnO2)‚ Cr2O3‚ and copper thermite (CuO). Aluminum can also be replaced by any reactive metal. Aluminum‚ however‚ is the safest material and the cheapest to use. Thermite‚ if you look
Premium Heat Fundamental physics concepts Electric charge
Determining the enthalpy change for the thermal decomposition of potassium hydrogen carbonate into potassium carbonate. Controlled Variables: 1. Volume of HCl ± 0.5 cm3 (± 2%) 2. Concentration of HCl‚ 3. Same mass of K2CO3 and KHCO3 within specified ranges of 2.5 – 3.0g and 3.25 – 3.75g respectively 4. Same calorimeter used i.e. polystyrene cup is used in this experiment 5. Same thermometer will be used ± 0.10K 6. Same source of K2CO3‚ KHCO3 and HCl Raw Data Results: The raw
Premium Thermodynamics Enthalpy Energy