measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the ONPG
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Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water
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There are COX enzyme isoforms include COX-1 and COX-2. (Figure 3.8) These enzymes originate from different genes and have distinct tissue-specific expressions and biologic functions. COX-1 is constitutively expressed in most tissues and believed to responsible for the production of prostanoids in homeostasis‚ such as gastric epithelial cytoprotection and renal blood flow maintenance. COX-2 expression is inducibly expressed only at marginal levels under condition in many tissues and is upregulated
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You will submit the completed chart and the reflection to your instructor. Human vs.________|# of differences in the amino acid sequence| Rhesus monkey|1| Horse|3| Donkey|3| Common zebra|11| Pig‚ cow‚ sheep|10| Dog|9| Gray whale|10| Rabbit|8| Kangaroo|10| Chicken‚ turkey|12| Penguin|12| Pekin duck|14| Snapping turtle|18| Rattlesnake|22| Bullfrog|26| Tuna|29| Screwfly fly|32| Silkworm moth|36| Tomato horn worm|37| Wheat|37| Rice|38| Baker’s Yeast|42| Candida Yeast|48| Neurospora|50|
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Stem cells are unique because they have the ability to generate new cells of any kind during early life and growth. There are about 200 different types of cells in the body; such as muscle‚ skin and brain cells. Most of these cells have to be repaired or replaced once in a while. For example our taste buds are replaced every 10 days‚ skin cells every couple of weeks and liver cells every 300-500 days. Stem cells serve as an internal repair system dividing essentially without limit to replenish
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Biology Enzymes IA Design Introduction: Enzymes are globular proteins‚ they are responsible for most of the chemical activities of a living organism. They act as catalysts‚ substances that affects the reaction of other substances without being destroyed or altered during the process. They are extremely efficient in the body system of living organisms‚ one enzyme may catalyse over a thousand chemical reactions every second. But there are certain conditions that need to be fulfilled in order for the
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Enzymes are catalytic proteins which speed up the rate of reactions. Every enzyme has a specific function – meaning‚ they can only bind to certain substrates. Because these enzymes are proteins‚ they can be denatured. Enzymes can be denatured by many factors‚ such as pH and temperature. This lab was divided into three parts which examined the effects of pH‚ enzyme concentration and temperature on the rate of which enzymes catalyze. The pH is an index of hydrogen ions. In acidic conditions‚ where
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LABORATORY REPORT Activity: Enzyme Activity Name: Daniel Franco Instructor: Professor Jennifer Frere Date: 03.08.2015 Predictions Sucrase will have the greatest activity at pH 6 Sucrase will have the greatest activity at 60 °C (140 °F) Sucrase activity decreases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of
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Cell Energy Worksheet Answer the following questions: Cellular respiration: • What is cellular respiration and what are its three stages? The main function of cellular respiration is to generate ATP for cellular work; it is the process of harvesting chemical energy from organic fuel and converting it to ATP energy. The three stages of cell respiration include: Glycolysis‚ Citric Acid Cycle‚ and Electron Transport(Simon‚ Reece‚ & Dickery‚ 2010). • What is the role of glycolysis
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