This experiment was performed in order to analyze the effects of pH‚ enzyme concentration‚ and temperature on the catalytic rate on enzyme ALP. The initial experiment was done to approximate the optimum pH for the activity of the enzyme. The hypothesis was that alkaline phosphatase activity will be critically affected by pH effects. The hypothesis was valid because the data shows that the pH values for the acidic‚ basic‚ and neutral tubes increased over time‚ absorbing at similar rates. The basic
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| [pic] Introduction Enzymes are catalysts which speed up a reaction by providing an alternative reaction pathway of lower activation energy. But they do not undergo permanent changes and so they remain unchanged even at the end of the reaction. Many enzymes consist of proteins and non-proteins called the cofactor. The intra- and intermolecular bonds that hold proteins in their secondary
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structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell and is named according to the substrate they specifically affect. An enzyme works best under optimal conditions pertaining to temperature‚ pH level and salt concentration1. In unfavorable conditions enzymes will become denatured and ineffective
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Description: A peroxidase enzyme‚ which was extracted from a brassica compestris (turnip)‚ is tested under various conditions in temperature‚ pH level‚ and competitive inhibitor (hydroxylamine). ABSTRACT: In order to determine the properties of an enzyme‚ a peroxidase enzyme was extracted from a brassica compestris (turnip) and tested under various temperatures‚ pH levels‚ and by a competitive inhibitor (hydroxylamine). The enzyme activity was measured in various ways depending on the activity
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should show a red/brick color after being placed in boiling water for three minutes‚ but if a negative reaction occurs we will get blue color or no change at all. Another test that we were introduced to was the iodine test‚ which is used to detect starch. A positive reaction would result in a blue/black color‚ where as a negative reaction would be an amber color. Then in order to find the remaining agents (peptide bonds) we used Biuret’s solution‚ a violet color is produced when there is a positive
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Enzymes are catalysts that speed up chemical reaction but are not themselves consumed or changed by the reaction. The cell’s biological catalysts are proteins. Proteins are made up of one or more polypeptide chains that are folded to make an active site‚ an area in which a material to be acted on by the enzyme‚ called the substrate‚ will fit. The temperature‚pH‚ the concentration of enzyme‚ and the concentration of substrate all affect the activity of the enzyme and the rate of the reaction. The
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process to anabolic hormones of the human body. Intrinsically they have like outcomes on the human body to anabolic steroids‚ triggering fast weight and force advances‚ but of a minor scale cause of the frequency limiting consequence produced by the enzyme adaptation. However‚ this theoretical explanation is thought-out somewhat outdated due to the expansions which have been done in supplement science. Epistane is amongst the hottest upscale steroids obtainable today‚ and it is achieving attention very
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Restriction Enzymes Restriction enzymes (also known as restriction endonucleases) are a group of bacterial enzymes which cut double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this
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Varibles that affect Enzyme Catalysis Reaction Rates Introduction Molecules are constantly moving in our bodies and in nature. When molecules move fast enough they collide into one another‚ allowing chemical reactions to occur. Factors such as temperature and concentrations can either help increase or decrease these reactions. (Jubenville.) Enzymes are known as catalyst because they are able to speed up reaction rates without being destroyed or altered. They are able to encourage chemical reactions
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Enzyme Reaction to Variable Elizabeth Alvidrez University of Texas at El Paso Abstract: An enzyme is a protein that serves as a biological catalyst (Denniston‚ 2007). Metabolic reactions happen with in cells. Enzymes are proteins that are used to speed up these reactions without being consumed by them (Mader‚ 2010). Catalase is a catalyst that digests potent hydrogen peroxide and converts it into H2O and O (Campbell Reese‚ 2008). The environment plays an important role
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