– Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the reaction tubes using a new micropipette tip for each transfer of DNA and enzyme (refer to figure
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color. Then in order to find the remaining agents (peptide bonds) we used Biuret’s solution‚ a violet color is produced when there is a positive reaction. Where as a light blue color is produced in the negative reaction. The overall goal of this experiment is to find three of the four macromolecules (proteins‚ lipids‚ carbohydrates) that you can find in everyday living organism. These macromolecules are the chain like proteins‚ carbohydrates‚ and nucleic acid. These macromolecules are made of smaller
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………………………………………………………………………………..9 Properties of Enzymes and Competitive Inhibitors. Abstract: Properties of enzymes were found in this experiment and some other factors‚ which affect enzyme activity. Enzymes are catalyst; they catalyze very specific reactions. Results relating to the active site of specific enzymes played a big role while performing this experiment. The purpose of this experiment was to fin how inhibitors affect enzyme’s activity by competing for the active
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Abstract The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor
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Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because we assume the more substrate an enzyme gets
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Exploring Enzymes - Ground-Up Tissue Activity Abstract Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined
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set: a) To analyze the decay of pressure after washing and to find out the steady pressure or mechanical properties after several washes. b) To analyze how different washing media: water‚ detergent and physiological saline affected the change in pressure. c) To find out the bias angle of an elastic fabric with which has the steadiest young’s mouduls d) To discuss if the decline of pressure is critical for the manufacturers to have pre-washing before making the pressure garment/girdle.
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Lab Ex#8: "Enzymes: Catalysts of Life" INTRODUCTION Enzymes are protein organelles where chemical reactions take place to generate energy within our cells. Without the energy produced from the cell enzyme activity‚ we would not possess the catalyst activity necessary for energy to produce movement. Each enzyme performs a specific function within our bodies. Those functions performed can be significantly altered with the introduction of variables outside their environment. Variables‚ such as temperature
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Miliaria or heat rashes. It is a skin disease marked by small and itchy rashes. When the sweat accrues in certain parts of our body and results in the formation of small red bumps‚ which causes irritation and severe itching‚ then trying out prickly heat powders is a very possible solution. Prickly heat mostly affects back‚ underarms‚ near waist area‚ and chest. Newborns and infants are predominantly vulnerable to prickly heat because their sweat ducts are not mature. This makes it easier for the beads of
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The purpose of this experiment is to study how enzyme activity is affected by environmental conditions. Researchers tested the level of potato extract enzyme activity with 1-11 pH‚ varying temperature‚ catechol solution‚ hydroquinone solution‚ and different measurements of catechol. In Figure 1A and 1B‚ pH levels were tested with potato extract to see how pH would affect the amount of Benzoquinone is formed in the potato. Although it was hypothesized that enzymes would form Benzoquinone better in
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