Abstract This experiment aimed to study the effect of various denaturants on albumin and casein protein extracts through viscosity measurements. 5 mL samples of native and denatured protein solutions were prepared‚ using -mercaptoethanol‚ urea and SDS as denaturants for albumin‚ and NaOH‚ NaCL‚ HCL‚ -mercaptoethanol‚ urea and SDS for casein. 5 mL blank solutions for each denaturant used were also prepared. The viscosity of the solutions were determined using Ostwald viscometer. ________
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fragmentation of the DNA. Protein glycosylation may be an additional damaging factor . In the attempt to repair DNA‚ poly(ADP-ribose) polymerase (PARP) is overstimulated. This diminishes cellular NAD+‚ and subsequently ATP‚ stores the depletion of the cellular energy stores ultimately results in beta cell necrosis. Although streptozotocin also methylates proteins ‚DNA methylation is ultimately responsible for beta cell death‚ but it is likely that protein methylation contributes to the functional defects
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Fiber Crops Production Jul 2nd‚ 2010 | Category: Farming & Production‚ Livelihood The Philippines is one of the world’s largest producers of fiber crops. It is blessed with optimal weather conditions‚ good types of soil‚ even distribution of rain throughout the year‚ and other related factors‚ which make growing fiber crops favorable. Abaca‚ cotton‚ maguey‚ pineapple‚ and ramie are just some fiber crops grown in the country. These crops are used in making different products sold in local and
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QUALITATIVE TESTS OF CARBOHYDRATES Final Lab Report Submitted by Brittany Fitzgerald 545301 Prepared for Nancy Cook Chemistry 3501 Monday November 26‚ 2012 RESULTS PART A: As a result of the Benedict’s test on various sugar solutions‚ it was found that galactose‚ mannose‚ arabinose‚ ribose‚ lactose‚ fructose‚ maltose‚ and cellobiose tested positively and therefore are considered reducing sugars. Glucose‚ starch‚ sucrose‚ and methyl-D-glucopyranoside on the other hand tested
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We have four important macromolecules; carbohydrates‚ lipids‚ proteins‚ and nucleic acids. From these four‚ the macromolecule I chose for this assignment was carbohydrate. Carbohydrates branch off into three main categories; monosaccharides‚ disaccharides‚ and polysaccharides. I decided to focus on disaccharides‚ specifically sucrose. Sucrose is more commonly known as table sugar. It has a chemical formula of C12H22O11. It is physically described as a white‚ odorless (although when heated‚ it may
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Practical 3: Proteins Introduction: ‘Protein’ also an important food component‚ we commonly take in from our diet. In this experiment we have studied a certain property of protein‚ i.e.‚ protein can be denatured by various factors. A. Denaturation of egg white Results: Egg white sample in water baths | Test tube | temperature | Observations | 1 | 60°C | Took 7minutes and 15 seconds to turn white and form white participate. | 2 | 80°C | Took 2minutes and 20 seconds to turn white
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DATE: 11/10/12 COURSE CODE: BIOL 2365 Comparative Biochemistry TITLE: Proteins and Amino Acids RESULTS: Table 1: The results of experiment 1; the Lowry Test Volume of Standard Protein/ Unknown (mL) Absorbance at 750 nm 0 0.000 0.1 0.017 0.3 0.135 0.3 0.155 0.5 0.230 0.7 0.323 0.7 0.310 1.0 0.457 1.0 Unknown 1a 0.463 1.0 Unknown 1b 0.433 1.0 Unknown 2a 0.237 1.0 Unknown 2b 0.159 Table 2: The results of Experiment 2; Ninhydrin Test Amino acid Color X
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“Fat Tax” Not a Panacea “Obesity now contributes to the death of more than 360‚000 Americans a year. The incidence of childhood obesity is now at epidemic level. Alarm bells are going off all over the place. But our government has done virtually nothing.”- Tom Harkin. Obesity is a medical condition in which excess body fat has accumulated to the extent that it may have an adverse effect on health‚ leading to reduce life expectancy and/or increased health problems (Wikipedia). To cut that number
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Proteins are polymeric chains that are built from monomers called amino acids. All structural and functional properties of proteins derive from the chemical properties of the polypeptide chain. There are four levels of protein structural organization: primary‚ secondary‚ tertiary‚ and quaternary. Primary structure is defined as the linear sequence of amino acids in a polypeptide chain. The secondary structure refers to certain regular geometric figures of the chain. Tertiary structure results from
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4A.2 RRL 4A.2.1 Coagulation of Proteins Coagulation of protein refers to sticking together‚ like a blood clot‚ usually as a result of denaturation or coming out of solution due to abnormal ionic strength or a change of solvent. Definite characteristics of the proteins are changed when they are coagulated‚ among which is loss of solubility in water and dilute salt solutions. In some instances and under certain conditions the coagulation process may be reversible. (Campbell‚ et.al‚ 1979) 4A.2.2
Free Hydrochloric acid Sodium chloride Egg white