LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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concentration of dilute solution V2 = volume of dilute solution Chemicals 0.100 M FeCl3 solution 0.100 M KCNS solution 0.005 M FeCl3 solution (B) Apparatus Burette 1 x 50 mL Pipette 1 x 5 mL Test tube 10 2.3: ACTIVITIES Method: 1. Based on given equation‚ calculate and prepare the following solution from standard solution of 0.100 M FeCl3 by using pipette. a. 25mL FeCl3 solution (5.0 x 10-2 M) b. 25mL FeCl3 solution (1.0 x 10-2 M) c. 25mL FeCl3 solution
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a) ‘Contact with other people is the most significant factor influencing an individual’s sense of belonging.’ To what extent is this view supported in your prescribed text and at least ONE other related text of your own choosing? Introduction Thesis: It is agreeable to a large extent that contact with other people significantly impacts an individual’s sense of belonging through connections and contrastingly‚ disconnections with the larger world Texts: This particular sense of belonging is evident
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Genotype is the genetic make-up of an organism while phenotype is the observable characteristics of an organism. The expression of a trait‚ phenotype‚ can be affected by the Sullivan 2 genetic constitution‚ genotype‚ and other genes and environmental factors. Mendel found this out after doing an experiment with peas. He
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Factors influencing the propensity to have extramarital affairs Introduction Marriage declares a legal and socially recognized monogamous relationship between two individuals that is binding for the duration of their life together. While the expectation for marriage is that the spouse will remain faithful to only their partner‚ relationships (whether romantic or sexual) which develop outside the bounds of marriage constitutes infidelity and are actually not uncommon. Extramarital affairs are not
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Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce
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I also hypnotized that the enzyme is acidic on the pH scale because it exists in our small intestine and our small intestine uses acid to break down food. The result from the lab prove my hypothesis correct because lactase worked well at body temperature and it also worked well at pH of 4.5 to 5.5 mg/dl proving that lactase works best at an acidic pH number. The lab shows that the highest amount of glucose is made by temperatures close to body temperature. In our our lab‚ we found that at 35 °C 500
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Introduction Enzymes are proteins that act as catalysts to regulate metabolism by selectively speeding up chemical reactions in the cell without being consumed during the process. During the catalytic action‚ the enzyme binds to the substrate – the reactant enzyme acts on – and forms an enzyme-substrate complex to convert the substrate into the product. Each type of enzyme combines with its specific substrate‚ which is recognized by the shape. In the enzymatic reaction‚ the initial rate of activity is constant
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and Hydroxylamine on the Enzyme Peroxidase Extracted From Brassica rapa Abstract In this experiment the enzyme peroxidase was extracted from from a turnip‚ Brassica rapa‚ and tested under different conditions. The effects of temperature‚ boiling‚ pH‚ and a competitive inhibitor were tested. The enzyme was tested at temperatures of 4°C‚ 24°C‚ 32°C‚ and 48°C. As the temperature increased‚ so did the activity of the enzyme. The enzyme was tested at pH levels of 3
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Maryely Ramirez Professor Theresa Sterner Bio Lab 105 04 November 2013 The Effects Environmental Temperature and pH have on the Activity of Porcine Pancreatic Amylase Introduction There are many types of enzymes and each has a specific job. Enzymes are particular types of proteins that help to speed up some reactions‚ such as reactants going to products. One of them is the amylase enzyme. Amylases are found in saliva‚ and pancreatic secretions of the small intestine. The function of amylase is
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