Formal lab report: Abstarct: The Purpose of this experiment was to perform Agrobacterium-mediated transformation in Wild type Arabidopsis thaliana Columbia by using somatic plant transformation method. The whole process lasted for over a period of 11 weeks and we were successful in getting transformed plants. Agrobacterium tumefaciens strain containing pMP90 (Ti-helper) plasmid and pCAMBIA1391 (T-DNA) plasmid was used for this plant transformation. pCAMBIA1391 plasmid was constructed by cloning Brassica
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Testable Question: What will happen to different halide salts found in the body if various compounds are added to the halide? How will the halides react? Introduction: For this lab we tested 6 halide salts found in the body‚ and their reactivity when a compound is added. We tested the halides NaF‚ NaCl‚ KBr‚ KI‚ and two unknown halides. To find the reaction of the halide‚ we are adding drops of Ca(NO3)2‚ AgNO3‚ AgNO3 + Na2S2O3‚ and NaOCI + Starch to the halides. Each halide will be separated from
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ions. Materials: Safety glasses 10 test tubes Test tube rack Paper Pencil 50 mL beaker Bunsen burner Nichrome wire Barium Nitrate Copper Nitrate Strontium Nitrate Lithium Nitrate Potassium Nitrate Sodium Chloride Calcium Nitrate Unknown solutions A‚B‚ & C Hydrochloric Acid Wash bottle (with distilled water) Procedure: 1. Label the test tubes with the names of the solutions and unknowns. 2. Place about 5 mL of each solution into each test tube‚ and 10 mL of Hydrochloric Acid into
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A flame test is conducted when an element is burned and the flame produces a color different the starting flame color. When the coffee stirrer was put into the flame‚ the element’s atoms (from the solution of the stirrer) left the original level of energy. When the atoms fell back to ground level‚ they released photons in the form of colored flame. Using the flame test‚ unknown compounds can be identified when they are burned since each element has a specific color. (Douma‚ 2008). The color
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Verifying Differences Between Athletes’ Heart Rates And Non-Athletes’ Heart Rates By Using Step Test Introduction: Different people have different heart rates because of many reasons such as health condition‚ smoking‚ working out‚ among others. This step test is used to compare and figure out the differences between athletes’ heart rates and non-athletes’ heart rates before and after the test. Kuno Hottenrott stated that the normal average heart rate of people ranges from 40 to 100 beats per
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Lab Questions: Are flame tests valuable for detecting metal ions present in a mixture of metal ions? Flame test may not be valuable for detecting individual metal ions present in a mixture of metal ions. Although each metal ion emits a different color of light‚ which corresponds to a certain wavelength of
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two plates will just contain Luria Broth. Once again‚ one will contain plasmid and one will not. The research question for this experiment is: What is the difference in the transformation efficiencies between pFLO and pBLU. Before completing this lab‚ a first trial was done following the same procedure below but instead of pFLO‚ pBLU was used. It is believed that the transformations efficiencies should be similar due to the fact that both plasmids are
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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Repeat step 4 for each of the remaining salts. 3. Repeat step 4 for each of the unknown metals and predict the identity of the unknown metals. Repeat any of the known metals to help with this prediction. **Wash hands thoroughly before leaving the lab. ** Some of the salts used are poisonous or irritating to the skin. Keep your hands away from your mouth until after you have washed them and have let the
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Discussion: There were some patterns that were noticeable during this experiment. It was noticed that all of the sprays with the metal potassium in them had turned the flame a light pink for a few seconds. The sprays that held the copper in them all turned the flame a vivid green colour‚ with some including green sparks also. Both of the sodium bottles turned the flame orange for a short time. Electrons orbit the nucleus of an atom in energy levels. If atoms are heated up to the point where the
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