Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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terms (excluding inflation) sales will slip very slightly. Q: Which segments have most potential for growth? A: There is a clear divide between two promising segments – liquid soaps and shower gels – and two less buoyant ones – bar soaps and bath additives. Mintel expects liquid soap and shower gel
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Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
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and placed in the thermal cycle. Gel electrophoresis is being run in order to analyze and compare the DNA samples at the crime scene with the guilty suspects. Gel electrophoresis is used to separate DNA using an electric current applied to the gel matrix‚ which causes the DNA samples to move towards the positive charge. The results obtain from the gel electrophoresis able to concluded the which suspect have been at the crime scene by analyzing against the
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nanodrop-spectrophotometer to determine concentration and purity of our product. Gel electrophoresis: We performed agarose gel electrophoresis to separate DNA fragments by size. Since DNA has a negative charge‚ it migrates towards the positively charged site presence of an electric current. Agarose solution is prepared by agarose powder and TAE buffer. Ethidium bromide (EtBr) which is kind of mutagen is added for track DNA on a gel. EtBr binds DNA and makes it visible under the UV light. Then‚ the electric
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Bio 1 Lab: Electrophoresis and DNA fingerprinting Jani Lynette Hagen October 31‚2014 U74644799 Electrophoresis is a technique which uses an electric field to separate molecules‚ allowing for identification and characterization of the molecules. It is commonly used to separate nucleic acids and protein molecules of various sizes. To prepare the gel for electrophoresis the amount of agrose needed must be calculated. For a 0.8 percent gel 0.8 grams of agrose is necessary per 100 ml of buffer. The DNA
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Purpose In this lab‚ we used PCR and gel electrophoresis to identify genetically modified food. Introduction A genetically modified organism is an organism whose DNA or genetic makeup has been modified to code for certain desirable traits("Genetically Modified Foods"). Common genetically modified plants include corn and soy‚ and common genetically modified animals are fish. Many genetically modified plants are coded to resist bugs‚ grow faster‚ and produce bigger fruit‚ while most GMO animals
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Relationship between Gene Copy Number‚ Amylase Concentration‚ and Gene Evolution Matthew Fantauzzi 400007178 Shawn Hercules - L15 25 November 2015 Abstract In this lab‚ students were experimenting to determine if a relationship exists between gene copy number‚ amylase concentration‚ and gene evolution. At the same time‚ this lab was designed to introduce university freshman to the etiquette and conventions used in a formal research setting. The methods used ranged from sample production‚ dilution
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September 23‚ 2012 Introduction: Bacteria are everywhere. Some can be seen with the naked eye and some require a microscope but how do we distinguish one kind of bacteria from another? To answer this question‚ we were required to complete three bacterial labs that helped us to understand what microorganisms are and how to identify and classify them. Thus‚ the main purpose of this project is to identify our unknown microorganisms‚ more specifically‚ our unknown bacteria. There are many ways to distinguish
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6 November 2012 SUBJECT: Lab Report Analysis This memo proposes the observations and claims I collected from reviewing three different lab reports. Three fields of study are composed within this memo that includes Electrical Engineering‚ Environmental Engineering‚ and Petroleum Engineering. “Electrical Filters‚” (Electrical)‚ written by Joe Schmoe‚ is a lab report made by a student at a university. The College Board produced an environmental lab report named‚ “Monitoring Air Quality‚”
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