Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of Michaelis-Menten Constant 7 3.7 Ammonium Sulphate Fractionation 7 3.8 Gel Filtration on Sephadex 7 3.9 Chromatography on DEAE-cellulose 7 4 Results 8 4.1 Freshly Prepared Crude 8 4.2 Crude Thawed after a Week of Freezing 9 4.3 Sephadex 25 Gel Filtration 11 4.4 Determination of Optimum pH 12 4.5 Ion Exchange Chromatography 13 4.6 Michaelis-Menten Kinetics 13 5 Discussion and Conclusion 14 6
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Chemistry: All About You Module 6. Hands on activities: Water experiments CONTENTS Module 6. Hands on activities: Water experiments ................................................................... 2 1. Filtration ............................................................................................................................ 3 2. Solar Still challenge .......................................................................................................... 5
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Jennie K. Daniels Organic Chemistry 2121 11 February 2014 Synthesis of Aspirin Salicylic acid derivatives‚ or salicin‚ are found in the bark of the willow tree. In the 5th century B.C.‚ Hippocrates ground the bark into a powder‚ and later‚ the Natives Americans chewed on the bark to alleviate fever and pain1. In the 19th century‚ a German chemist by the name of Felix Hoffman wanted to find a medication that would ease
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such as extraction‚ evaporation‚ and filtration‚ the three components will be isolated based on their solubilities and acid-base properties. The percent composition of Panacetin will also be deduced based on the masses of the three dried components; this is done to verify the composition attained is consistent with those listed on the preparations label. As a result of this investigation‚ my teammates and I allowed the Panacetin to undergo gravity filtration and separation techniques in order to
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Recrystallization and Melting Point of Benzoic Acid Organic Chemistry 1 10/4/12 Abstract Recrystallization was done to remove impurities from the sample. The percent recovery of benzoic acid during recrystallization is 23.02%. The difference between the pure and impure samples was observed by comparison of melting points. It was found that impure sample had a lower and wider melting point range of 120.1-122.2 (C). The pure sample melting point range was 121.3-122.5 (C). These ranges helped determine
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reduce cerebral edema to decrease ICP. Mannitol mechanism of action is to reduce ICP by drawing water from the brain parenchyma‚ does not cross brain-blood barrier (BBB). But also causes profound osmotic diuresis by increases the osmotic pressure of glomerular filtrate‚ inhibits tubular reabsorption of water and electrolytes‚ increases urinary output if renal failure or hypovolemia is a concern could be devastating to patient’s outcome. HTS mechanism of action is the same osmotic gradient in the BBB
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Melting point 1. What two effects do impurities have on the melting point of an organic compound? Soluble impurities affect the melting point of a solid in the following manner. a. The presence of an impurity in the molten compound reduces its vapor pressure thus lowering the melting point of the compound. b. Broaden the melting point range. 2. For what tow purpose are melting points routinely used a. To determine the identity of an organic solid. b. To determine the purity of an organic solid
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Experiment #1 Nucleophilic Aromatic Substitution of 2‚4-dinitrochlorobenze Name: Anouk Deck-Leger Student I.D: 9380868 Date performed: September 13th‚ 2010 Due Date: September 20th‚ 2010 Introduction: The company DNCB produces large amounts of 2‚4-dinitrochlorobenzene and they sell this product to treat against warts and severe and chronic hair loss. It can also be used as an alternative treatment for HIV. The supervisor notices an excess amount of m-aminobenzoic acid stored away which
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possibilities. Through the use of a vacuum filtration system the mass of Cu was found to be 2.1726g which meant that through limiting reagent analysis Fe was determined to be the limiting reagent and the chemical reaction was determined to be as following:- CuSO4(aq) + Fe(s) Cu(s) + FeSO4(aq) Introduction: There were several purposes of this lab‚ the main ones were limiting reagent analysis‚ quantitative and analytical analysis‚ and techniques like vacuum filtration and weighing by difference. The main
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Activity 1: Stimulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. The two variables that affect the rate of diffusion are: • The composition of the lipid layer‚ this content varies from tissue to tissue. For example kidney tissues have a high cholesterol content which makes them impermeable to water. • The size of the molecule‚ the larger the molecule the slower the rate of diffusion. 2. Why do you think the urea was not able to diffuse
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