battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to be identified; the basic ideas and practice of identification of an unknown sample of bacteria are important for a microbiologist to develop. Not only is proper procedural practice
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different bacterial species have been isolated from fish-processing environments‚ e.g. Exiguobacterium spp. has been isolated from a fish processing plant in Japan (Yumoto et al.‚ 2004) and Staphylococcus spp. in food is often associated with human contact. Furthermore‚ due to the halophilic nature of Staphylococcus spp. (Simon
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A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from
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Aims The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different
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University Of Botswana DEPARTMENT OF BIOLOGICAL SCIENCES BIO111: Laboratory Exercise 8 The Mutagenic Effects of UV Light Name: sharon reakae INTRODUCTION A mutation is a permanent change in the DNA sequence of a gene. Mutation rates are usually very low but there are factors that can increase the mutation rates. These factors are referred to as mutagens and conclude a range of physic-chemical agents. UV light is a mutagen that affects all living things and has an adverse effect on the
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Microbial nomenclature: The name of the bacterium should written and spelled correctly. The name should be italicized or underlined. Italicized is preferred. For example‚ Staphylococcus aureus. The genus is capitalized but the species is not. After the full genus name is given in the paper‚ it can be written as S. aureus‚ but still italicized. This is as long as there in no other genera in the paper that starts with the same letter. PARTS TO THE UNKNOWN LAB REPORT (Note: Other than the
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two main micro-organisms that cause TSS are Staphylococcus aureus and Streptococcus pyogenes. TSS is associated with the use of highly absorbent tampons but can also arise from Staphylococcus aureus infection at non-genital areas (for example wounds).[4] "From a UK population of around 60 million there were about 40 cases reported each year‚ half of which are associated with women using tampons." [1] Staphylococcus aureus are anaerobic‚ gram positive bacterium. They lie harmlessly in the
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Some important examples include penicillin-resistant Streptococcus pneumoniae‚ vancomycin-resistant enterococci‚ methicillin-resistant Staphylococcus aureus‚ multi-resistant Salmonella typhi‚ Shigella dysenteriae‚ Neisseria gonorrhea‚ Pseudomonas aeruginosa and multi-resistant Mycobacterium tuberculosis. The development of resistance to drugs commonly used to treat P. falciparum malaria is of particular concern‚ as is the emerging resistance
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plate seemed to show that E. coli did not have lipases because it did not have clear zones. Staphylococcus aureus seemed to have these clear zones meaning it can secrete lipases. The bacteria Bacillus subtilis did not grow well to see if there was a clearing. After doing some research‚ it was shown that B. subtilis is lipase positive so there should have also been some clear sones like the Staphylococcus aureus (Watson). The DNAse agar could not have been analyzed because HCl was not available to precipitate
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Another sample was applied to a glass slide‚ heat fixed and a Gram stain test was performed. From the list of several possible choices for the unknowns‚ about half are eliminated. Table 1 indicates the tests that were performed on the unknown sample‚ all materials required for each test‚ as well as factors determining a positive or negative result. Test Performed Media/Reagents Used Positive Test Observations Negative Test Observations Gram Stain Crystal violet Gram’s iodine 95% Alcohol
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