Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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Abstract: Introduction: Materials: * Chemicals: Buffer solution‚ pH 7.0‚ 50 mL Phenolphthalein indicator solution‚ 1.0 %‚ 1 mL Potassium hydrogen phthalate‚ KHC8H4O4‚ 2 g sodium hydroxide solution‚ NaOH‚ 0.1 M‚ 150 mL Unknown weak acid‚ 1.5g Water‚ distilled or deionized * Equipment: Balance Stir bar Beaker‚ 250mL Oven Buret‚ 50 mL pH sensor Desiccator Rising stand and buret clamp Erlenmeyer flask‚ 125mL Wash bottle with distilled water Funnel Weighing dishes‚ 2
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Experiment Acids‚ Bases‚ and Neutrals Problem Are common house-hold products pH balanced and neutral? Information Gathered pH measures how acidic or alkaline a solution is. The pH scale ranges from 0-14. Solutions that range from 0-6 are considered to be acidic. Solutions that are on the acidic end of the scale are low in pH‚ high in hydrogen ion‚ and low in hydroxyl ions. An acid has a sour taste‚ hence‚ will react to metal and will be corrosive; therefore‚ they are considered as an electrolyte
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9.1 Sulphuric Acid Sulphuric Acid is Manufactured in Industry 1. Sulphuric acid‚ H2SO4 is manufactured in industry through Contact Process. 2. The raw materials used are sulphur‚ air and water 3. The Contact process consists of three stages. Stage 1: The production of sulphur dioxide This can be obtained by two methods: 1. Burning of sulphur in dry air in the furnace S + O SO2 2. Burning of metal sulphide such as zinc sulphide or iron(III) sulphide in dry air. 2ZnS
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STANDARDIATION OF ACID AND BASE Eunice Ivy B. Gamboa ABSTRACT Solutions of known concentration are prepared by dissolving measured masses of standard acids in distilled water. The concentrations of unknown solutions of sodium hydroxide are determined by titration. An acid solution reacts with a base solution in a "neutralization" reaction. Titrations permit the concentrations of unknown acids/bases to be determined with a high degree of accuracy. In order to analyze unknown acids/bases‚ we must have
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Oxalic Acid Lab Aim: Use acid base titration to determine the number of water molecules in hydrated hydrochloric acid. Apparatus required: Oxalic acid solution 250 cm3 Weighing bottle Digital balance Beaker (250 cm3) Distilled Water Volumetric Flask 250cm3 Filter funnel Pipette Burette 50cm3 Retort Stand Beakers 100cm3 Standardized sodium hydroxide solution 0.1M Pipette filter Conical flasks 250cm3 Phenolphthalein Indicator Procedure 1) Rinse the burette with distilled
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EXPERIMENT 2 ACID DISSOCIATION CONSTANT OF AN INDICATOR DYE OBJECTIVES Using spectrophotometric method: determine the wavelengths at which the acid and base forms of the dye in aqueous medium exhibit maximum absorption; determine the molar absorptivities of the acid and base forms of the dye and estimate an unknown concentration of the dye in solution using the Beer-Lambert’s Law; and determine the acid dissociation constant of the indicator dye. THEORY The absorption or reflection of
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Tests for Carboxylic Acid and Derivatives Mary Catherine Sarte‚ John Emmanuel Sy‚ Allurie Umel‚Franklin Yap‚ Mary Christine YouIntroduction Carboxylic acids derivatives are simply groupsof compounds that contain a carbonyl group butwith an electronegative atom attached to thecarbon. The difference in the structure leads to amajor change in reactivity. The reactions of thesegroups of compounds involve nucleophilicsubstitution. Although there are abundant kindsof carboxylic acid derivatives‚ the experimentonly
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expected melting point. After its temperature reached the plateau‚ the temperature increment was set at 1°C per minute‚ and the melting point range of 152°C-154°C was recorded. After standardizing the melting device‚ a small sample of unknown carboxylic acid was placed in a capillary tube‚ and an arbitrary plateau of 200°C was set. When the sample started to melt at 150°C‚ another capillary tube containing a small amount of unknown sample was placed in the melting device‚ and the plateau was set to 135°C
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Writing a Synthesis Essay/Paper A synthesis essay draws on two or more sources and combines their ideas into a coherent whole. The word "synthesis" means to combine related things in a meaningful way‚ with a specific purpose in mind. When you synthesize‚ you blend the parts in such a way that you create something that is worth more than merely the sum of the parts. For our purposes‚ synthesis means putting together the arguments of various authors in a way that sheds new light on the discussion
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