Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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The study of shelf life for liquid biofertilizer from vegetable waste showed that contrary to the traditional advice that liquid biofertilizers produced from fermentation by effective microorganisms‚ should be used within three months of production‚ the liquid biofertilizer produced from vegetable waste still contains high amount of viable microbial population after four months of storage with no significant effect of the presence or absence of light (Ngampimol and Kunathigan‚ 2008). The most common
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Unversity Visca‚ Babay City‚ Leyte Name: Millama‚ Engene O. Course & Year: BS Biotech-2 Lab Schedule: W (1-4) Group 5 Date Performed: Dec. 12‚ 2012 Date Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment exists in two forms; chlorophyll a and chlorophyll b. Chlorophyll a‚ being
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Post Lab #4- Column Chromatography Organic Chem 3418-2 March 3‚ 2011 Theoretical Background- The fluorene and fluorenone mixture was separated by first dissolving the mixture in heptane. Since “like dissolves like”‚ fluorene dissolves with the non-polar heptane and the polar fluorenone dissolves in the polar ethyl acetate solvent. This phenomenon was illustrated in class before the experiment‚ when it was pointed out why water will not dissolve fluorene‚ fluorenone‚ or transstilbene
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Liquid Nitrogen Randi Pinter Community College of Aurora Rebecca Hoenigman Chem 101-360 Sitting in a classroom with a guest speaker‚ wondering what was in the tank and why there were carnations nearby. As a young student‚ guest speaker days were an exciting thing to be a part of. The full history of the lesson may have been lost in time; but when the speaker dipped a carnation in a tub of liquid that was pouring smoke onto the classroom floor‚ the results of his actions had the power to make a
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Global Liquid Elevator and Pump Market to 2019 - Market Size‚ Growth‚ and Forecasts Released On 16th September 2015 This comprehensive publication enables readers the critical perspectives to be able to evaluate the Global market for liquid elevators and pumps. The publication provides the market size‚ growth and forecasts at the global level as well as for the following countries: Argentina‚ Armenia‚ Australia‚ Austria‚ Azerbaijan‚ Belgium‚ Bulgaria‚ Canada‚ Chile‚ China‚ Colombia‚ Czech Republic
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Surface Tension Properties of Liquids Introduction The purpose of this experiment is to measure and record the surface tension of water and other aqueous solutions using capillary rise method techniques and practicing lab safety. In this laboratory the surface tension of water depends on the concentration of the following solutes; NaCl‚ acetone‚ and sodium dodecyl sulfate (SDS). Intermolecular interactions as well as other phases (solid‚ or air) make a liquid exist. The data collected will show
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RADICAL HALOGENATION AND GAS CHROMATOGRAPHY Abstract In radical halogenations lab 1-chlorobutane and 5% sodium hypochlorite solution was mixed in a vial and put through tests to give a product that can then be analyzed using gas chromatography. This experiment was performed to show how a radical hydrogenation reaction works with alkanes. Four isomers were attained and then relative reactivity rate was calculated. 1‚1-dichlorobutane had 2.5% per Hydrogen; 1‚2-dichlorobutane had 10%; 1‚3-dichlorobutane
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S. Larkai (2011 -2012 ) ‚ Principles of separation and reaction laboratory booklet : continuous stirred tank reactors . pp 1 – 4. Faculty of engineering science and the built environment‚ London south bank university. F.A.Holland and F.S.Chapman "Liquid Mixing and Processing in Stirred Tanks"‚ p.109 (Chapman & Hall‚ 1996). J.J Carberry. "Chemical & Catalytic Reaction Engineering"‚ p.92 (McGraw-Hill‚ 1976). J M Coulson & J F Richardson‚ Chemical engineering 2005. Atkins. P and Jones.L (2010) Chemical
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