The spectrophotometer is an essential tool for biologists and chemists in analyzing chemical and biological samples. Gaining familiarity with its operating protocols and understanding what its outputs mean are very important in the development of lab technique for students of cell biology. This experiment will help laboratory students gain experience in using the spectrophotometer. Basic Laws of Light Absorption - For a uniform absorbing medium (solution: solvent and solute molecules that absorb
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Extraction and Quantitative Determination of B-Carotene Introduction The study of B-Carotene is important because it is good for vision‚ immunity‚ and overall health. The purpose of this experiment is to extract and find quantitative amount of Beta-carotene from a carrot sample. The raw product(carrots) was extracted using simple distillation. The purity of the product will be characterized using percent yield‚ thin layer chromatography‚ Beer’s Law‚ and UV-vis. Beer’s law is used because in this
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In experiment 3.1.We set up and calibrate the spectronic 20 first since it takes time to be ready to use so‚ we turned the spectronic 20 to warm up for at least 15 minutes‚ set wavelength to 360 while no test tube in it. Also‚ we set the filter lever in order to match the wavelength range‚ and then set the transmittance to 0%‚ then we changed the mode to absorbance. After we labeled two test tubes which were #5 and #10‚ we inserted water first to set 0 absorbance‚ then we used #5 sample to read it
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The data we collected and the calculations we performed demonstrated that the color of a given food dye depended on which light was transmitted in the visible spectrum and which light was absorbed. A quantitative measurement of the absorbance spectrum of each food dye was obtained through spectrophotometry.1 Through this method‚ we determined that the wavelength of maximum absorbance of a given food dye was directly correlated to the color of that food dye. Further‚ darker food dyes absorbed more
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Methods We began this experiment by turning the Spectrophotometer to 605 nm absorbance‚ and setting up each of the tubes as stated in Table 7-2*‚ ignoring the DPIP until the rest of the sample was set up. Once all of the samples were set up accordingly‚ we blanked the spectrophotometer‚ and added the DPIP to the sample right before‚ inverting the sample and taking a reading at the Zero time mark‚ and placing the sample in the designated light apparatus‚ Sample 1 was wrapped in aluminum foil to
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1. The standard curve is a graph that shows a relationship between concentration and absorbance of a solution. A standard curve was experimentally created in this experiment using 10mL solutions of phenol red with concentrations 10µM‚ 7.5 µM‚ 5.0 µM and 2.5 µM then the absorbance of each sample was measured using a spectrophotometer. This generated curve with resulting average absorbances of 1.273nm‚ 1.0275nm‚ 0.585nm‚ 0.285nm and 0.124nm provided a means to determine the phenol red concentrations
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Observing Flames and Specs through a Spectroscope By: Seif Alkhouri Cristian Plancia-Mata Charlotte Maxwell Suryansh Kumar Evan Hatton J. Immel Ph.D Introduction Spectroscopy is the study of the interaction between matter and radiated energy. English philosopher Roger Bacon(1214-1294) was the first person to recognize that if sunlight passes through water it splits into colors. Around four centuries ago‚ Isaac Newton originated the title “spectrum” to label the shade of rainbows put
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Infrared thermometers are used to measure: Surface temp Internal temp of hot food Air temp Internal temp of frozen food 2. Food cannot be re-frozen once it is thawed because: re-freezing encourages dangerous bacteria growth dangerous bacteria can grow on thawed food dangerous visuses can grow on thawed food re-freezing ruins the flavor of the food 3. What is the min temp for transporting hot food and hot holding food items? 135+ 4. The person most likely to contract an illness caused
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survivors and deliver a small package such as a communication device or location marker to help rescuers determine victim’s location and condition. Solutions -Robot tethered through serial cable and controlled via a PS3 controller -Robot consists of infrared sensor to locate victim and proximity sensor to monitor distance between robot and survivor. I. Requirement and Stakeholder Analysis a. Many parties are involved in the construction‚ testing‚ and operation of the USRR‚ and thus a stakeholder
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According to R. James Seffrin‚ a director for the Definitive Internet Resource for Infrared Thermography‚ Seffrin explains that all objects emit infrared radiation as long as the object is above absolute zero (Kelvins). The cameras that can see and distinguish temperature are called‚ “thermal imagers.” These imagers convert the perceived radiation into scaled colors
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