Enzyme kinetics Purpose: The goal of this investigation was to measure the amounts of products made and see the different elements that that affect the rate of breakdown of p-Nitro phenol in the absence or presence of cellobiase….. Methods: Activity #1 The materials used for this activity are as follows: 1.5 mM substrate‚ enzyme‚ Stop solution‚ buffer‚ DPTPs‚ 15 ml conical tubes‚ cuvettes‚ marker‚ beaker‚ distilled water‚ spectrophotometer‚ stop watch. First four 15 ml conical tubes
Premium PH Enzyme Concentration
Enzymes are important components of life‚ facilitating reactions that are necessary for an organism to live. Enzymes can be very specific to what environment they function best in1. Numerous environmental impacts were tested for the enzyme peroxidase which catalyzes the decomposition of hydrogen peroxide. The basic decomposition reaction was carried out first without any environmental alterations. The hypothesis for this reaction was supported. The enzyme caused the amount of absorbance increase
Premium Enzyme Chemical reaction Catalysis
PNGase F Procotol Reactions may be scaled-up linearly to accommodate larger amounts of glycoprotein and larger reaction volumes. Optimal incubation times may vary for particular substrates. Typical reaction conditions are as follows: Denaturing Reaction Conditions: 1. Combine 1-20 µg of glycoprotein‚ 1 µl of 10X Glycoprotein Denaturing Buffer and H2O (if necessary) to make a 10 µl total reaction volume. 2. Denature glycoprotein by heating reaction at 95°C for 5 minutes. 3. Chill denatured glycoprotein
Premium Chemical reaction Enzyme Chemical equilibrium
How is pH affecting catalase activity? Hypothesis The catalase activity is assumed to be the most active in pH7. The higher or lower concentration away from the optimum pH of catalase‚ the slower the rate of activity is. Data Collection & Processing I collected the results of pH4‚ pH7‚ pH10 and pH13 after 2 minutes‚ and I repeated the experiment 3 times. Below is a table to show the results‚ their averages and standard deviations: | 1st time (sec) | 2nd time (sec) | 3rd time (sec)
Free PH Buffer solution Standard deviation
NaCl Effects on Peroxidase Activity My experiment was to see if adding NaCl to solution would have any effects on peroxidase activity. The materials that were used in this experiment were pH 7 buffer(DI water)‚ peroxidase‚ NaCl‚ guaiacol and hydrogen peroxide; added in that order. Blanks were created for each NaCl concentration‚ 0%‚ 5%‚ 7.5% and 10%. Each cuvette had .5ml of pH 7 buffer‚ 1ml of peroxidase‚ .02ml guaiacol for the experimental cuvettes and 0ml of guaiacol for the blank cuvettes‚
Premium PH Enzyme Chemistry
Did different conditions affect the Enzyme’s Efficiency? The hypothesis was “the more the imposed condition affects the mobility of the Enzyme‚ the fewer toothpicks will be broken”. This hypothesis proved true. This experiment manifested through multiple trials that the condition the enzyme had to endure definitely affected the Enzyme’s Efficiency. During this modeling experiment‚ it was observed that that the efficiency of the Enzyme over time created a line like that of a radical function
Premium Enzyme Chemical reaction Metabolism
The pH is another important factor that influences the enzyme activity‚ as it affects the shape of the enzyme or charge on substrate and ultimately its functionality (Reece et al.‚ 2011; Eed and John‚ 2013). With respect to effect of pH on enzyme activity‚ there was a steady increase in the activity for CFR15-protease‚ i.e. from pH 3.5 to 10.5 and a sudden fall was observed over pH 10.5. The activity was stable in the range of pH 7 to 10.5 and this reveals the alkaline protease nature of the enzyme
Premium Enzyme Chemical reaction Catalysis
Purpose: The purpose of this lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will
Premium Chemistry Chemical reaction Enzyme
Jay A. Torres With David Shbeeb and Matt Shinsky Liberty University 1003 Misty Mountain road apt.507‚ V.A. 24502 jtorres99@liberty.edu words count: 1‚313 Determination of Km and Vmax of Alkaline Phosphatase Graphs will be used with the enzyme alkaline phosphatase of the unknown in the enzyme solution to Determine Km and Vmax To Dr. Kimberly A. P. Mitchell‚ Editor‚ Liberty Journal of Cell Biology‚ 1971 University Blvd‚ Lynchburg‚ VA 24502. Materials and Methods Dilution: Alkaline Phosphatase
Premium Enzyme Chemistry Chemical equilibrium
Results Figure 1 shows that the response of the tissue increased with bath concentration of ACh (M). The logarithms of bath concentration for each response were used to form a clear sigmoidal formation‚ and thus depict a more clear relationship between concentration and response. Figure 1 shows the increasing rate of response as bath concentration increased‚ before plateauing around the highest concentrations. Responses ranged from 0.685-100%‚ while bath concentrations of ACh ranged from 2 x 10-10
Premium Solution Pharmacology Concentration