Abstract There are many methods employed to precipitate proteins out of solution. In this experiment we manipulated many physical and chemical variables in order to achieve purification of a protein via precipitation. In the first part of the experiment we purified the protein casein by modifying it’s pH. In the second part of the experiment we manipulated the ionic strength of albumin in egg whites‚ in a process called salting out. By manipulating these chemical properties we were able to
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2a: Infrared Spectroscopy and Analgesic Drug Identification Abstract: To be able to understand how organic soluble compounds could be isolated from non-organic components‚ a chromatographic separation process was used. The objective of the analgesic drug identification is to isolate the organic compounds/ active ingredients in an unknown drug. The identity of the drug was determined through melting point‚ percent recover‚ and Rf values. The comparable data for acetaminophen from the manufacturer
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solvent polarity and temperature will also be looked at. Name Structure M.W. M.P. B.P. Density Toxicity Sodium Iodide NaI 149.89 g/mol 661ºC 1304ºC 3.67 g/cm3 Yes Silver Nitrate AgNO3 169.87 g/mol 212ºC 444ºC 4.35 g/cm3 Yes Acetone C3H6O 58.08 g/mol -95ºC 56ºC 0.791 g/cm3 Yes Ethanol C2H6O 46.07 g/mol -114ºC 78ºC 0.789 g/cm3 Yes 1-Chlorbutane C4H9Cl 93.57 g/mol -123ºC 79ºC .88 g/cm3 Yes 1-Bromobutane C4H9Br 137.02 g/mol -112ºC 101.4ºC 1.26 g/cm3 Yes
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Experiment #10 & #11 The Synthesis of Cobalt Oxalate Hydrate The Synthesis of a Nitrite Complex February 5‚ 2012 Chemistry 1211L - 146 - Spring 2012 Procedure The Synthesis of Cobalt Oxalate Hydrate Place 100 ml of distilled water in a 250-ml (or 400-ml) beaker. Add 1.26g of oxalic acid dihydrate (H2C2O4.2H2O) and 1 ml of concentrated ammonia. Stir the mixture until the solid has dissolved completely. Dissolve 2.34 g of cobalt chloride hexahydrate (CoCl2.6H2O) in 100 ml of water
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student)‚ 30% acetone in heptanes (3 mls/student)‚ 95% ethanol (20 mls/student) wk 4: 1‚3 diphenylacetone (0.5 g/student)‚ triethylene glycol (5 mls/student)‚ 40% benzyltrimethylammonium hydroxide/methanol (0.5 mls/student) Part A: Thiamine catalyzed condensation benzoin condensation of benzaldehyde Procedure: (Week 1) Dissolve
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the purpose of iodine in the Gram’s stain procedure? (What is a mordant?) The iodine helps set the stain by forming an insoluble crystal violet-iodine complex. Basically helps retain the crystal violet to the cell. E. What is the purpose of acetone-alcohol in the Gram’s stain procedure? It is a decolorizer that differentiates between gram-negative and gram-positive. Only the gram-positive bacteria will retain the crystal violet-iodine complex. Gram-negative bacteria will not retain the color
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bristle)‚ and colors (metallic‚ non metallic‚ or ultraviolet reactive). Like spray paint these markers give off volatile organic compounds. Permanent marker can be removed using a dry erase marker on certain surfaces such as a whiteboard as it contains acetone. Alcohol based markers such as Sharpies are said to be safer‚ with water-based markers being the safest of all. The popular permanent markers are Sharpie‚ Stanford king size permanent marker‚ Marks a lot and Redi Mark +. Ingredients in the permanent
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(Answers to Questions) Q1. State what types of inetmolecular forces are present in solutions formed due to intermolecular attractions between the solute and the solvent. A1. For Water-Soluble Compounds: Acetone – Water: Hydrogen bonding and van der Waals forces Acetone – Diethyl Ether: Dipole – (induced) dipole and van der Waals forces Sucrose – Water: Hydrogen bonding and van der Waals forces Ethyl alcohol – Water: Hydrogen bonding and van der Waals forces Ethyl alcohol – Dietheyl
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Analysis of Alcohols‚ Aldehydes and Ketones Karl Wayne Mancao‚ Raphaell Mordeno‚ Andres Pastrana III*‚ and Shannen Peñaverde Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The proponents have done several tests for identifying alcohols‚ aldehydes and ketones. These tests are Dichromate test‚ Tollens test‚ Lucas test‚ DNPH test and Iodoform test. Three samples got positive result in dichromate test and one in Tollens test. Lucas test got one sample that has
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Hammer‚ face helmet (or goggles) and mask‚ rock powder container‚ plastic bag‚ spoon‚ gloves‚ labelling sticker and pen‚ cleaned Jaw crusher and TEMA Mill (i.e. laboratory disc mill). Material: Sample rock of Granite and Sandstone (hand specimen)‚ Acetone. Procedure: 1. A piece of rock is selected (based on required size)‚ cleaned and dried. 2. Using a sledgehammer‚ the selected rock sample is knocked into an approximately 5 to 10 cm in size (pebble-sized). For a hard rock (i.e. granite)
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