intermittently to ensure proper interaction between substances. Then it is cooled again to precipitate the product and separate it from some of the impurities. For further purification the product is recrystallized. A solubility test was performed on water‚ acetone‚ and methanol to pick a solvent for this process. For a substance to be optimal for recrystallization‚ the product has to be insoluble at room temperature and soluble at the solvent’s boiling point1. The selected solvent will allow everything to dissolve
Premium Chemistry Solid Water
EXERCISE 3: DIFFERENTIAL PROTEIN PRECIPITATION Vanessa Andrea Estepa University of Mindanao‚ College of Science and Mathematics ABSTRACT: Denaturation is the disruption in the original conformation of the protein wherein the secondary‚ tertiary and quaternary structures are all affected. Denaturation is brought about by various kinds of physical and chemical means; this includes the addition of strong acids‚ heavy metal cations‚ alkaloidal reagents‚ salting out and addition of organic solvents.
Premium Protein Amino acid Acid
2a: Infrared Spectroscopy and Analgesic Drug Identification Abstract: To be able to understand how organic soluble compounds could be isolated from non-organic components‚ a chromatographic separation process was used. The objective of the analgesic drug identification is to isolate the organic compounds/ active ingredients in an unknown drug. The identity of the drug was determined through melting point‚ percent recover‚ and Rf values. The comparable data for acetaminophen from the manufacturer
Premium Paracetamol Solvent Acetic acid
solvent polarity and temperature will also be looked at. Name Structure M.W. M.P. B.P. Density Toxicity Sodium Iodide NaI 149.89 g/mol 661ºC 1304ºC 3.67 g/cm3 Yes Silver Nitrate AgNO3 169.87 g/mol 212ºC 444ºC 4.35 g/cm3 Yes Acetone C3H6O 58.08 g/mol -95ºC 56ºC 0.791 g/cm3 Yes Ethanol C2H6O 46.07 g/mol -114ºC 78ºC 0.789 g/cm3 Yes 1-Chlorbutane C4H9Cl 93.57 g/mol -123ºC 79ºC .88 g/cm3 Yes 1-Bromobutane C4H9Br 137.02 g/mol -112ºC 101.4ºC 1.26 g/cm3 Yes
Premium Chemical reaction Ethanol Alkene
Danny Fish 10/9/11 Chemical Testing To identify An Unknown The hypothesis tested was that depending on the solution presented‚ which would test positive for one of the following‚ proteins‚ carbohydrates‚ or lipids through use of chemical testing. (Sudan IV‚ Benedicts’ Solution‚ Iodine‚ Biuret’s) . In order to gain more information for the hypothesis‚ one must know how to test for said macromolecule. Each of the above stated molecules has their own individual solution that will in turn identify
Premium Purple Blue Glucose
FEASIBILITY OF PRUSSIAN BLUE POWDER (TINA) AS PIGMENT FOR WHITEBOARD INK An Investigatory Project Presented to the Science Department Paliparan II National High School – Congressional Annex Brgy. Paliparan II‚ Dasmarinas City‚ Cavite By: Hidalgo‚ Briggs Lansang‚ Marinella Ranoco‚ Jessa Lonosa‚ Jamaicah Lumapas‚ Christopher Lawrence March 2014 Acknowledgement Abstract TABLE OF CONTENTS LIST OF APPENDICES Appendix 1. Sample Research Instrument Appendix
Premium Pigment
Bacterial Fermentation Secondary article Article Contents Volker Mu¨ller‚ Ludwig-Maximilians-Universita¨t Mu¨nchen‚ Munich‚ Germany . Introduction Under anaerobic conditions‚ in the dark and in the absence of electron acceptors‚ organic compounds are catabolized by strictly anaerobic or facultatively anaerobic bacteria by internally balanced oxidation–reduction reactions‚ a process called fermentation. In fermentation‚ the organic compound serves as both electron donor and acceptor‚ and adenosine
Free Cellular respiration Adenosine triphosphate
GROUP 2 EXPERIMENT 15 BSP2M ORGANIC CHEMISTRY LABORATORY MR. WILBERT MORALES Chemical Structure Physical Properties Hazards Acetonephenone Physical state and appearance: Liquid. (Liquid.) Odor: Not available. Taste: Not available. Molecular Weight: 120.16 g/mole Color: Colorless to light yellow. pH (1% soln/water): 7 [Neutral.] Boiling Point: 201.7°C (395.1°F) Melting Point: 19.7°C (67.5°F) Critical Temperature: Not available. Specific Gravity: 1.03 (Water
Premium Vapor pressure Temperature Water
Abstract There are many methods employed to precipitate proteins out of solution. In this experiment we manipulated many physical and chemical variables in order to achieve purification of a protein via precipitation. In the first part of the experiment we purified the protein casein by modifying it’s pH. In the second part of the experiment we manipulated the ionic strength of albumin in egg whites‚ in a process called salting out. By manipulating these chemical properties we were able to
Premium Water Protein Chemistry
the purpose of iodine in the Gram’s stain procedure? (What is a mordant?) The iodine helps set the stain by forming an insoluble crystal violet-iodine complex. Basically helps retain the crystal violet to the cell. E. What is the purpose of acetone-alcohol in the Gram’s stain procedure? It is a decolorizer that differentiates between gram-negative and gram-positive. Only the gram-positive bacteria will retain the crystal violet-iodine complex. Gram-negative bacteria will not retain the color
Free Bacteria Staining Gram staining