temperature for these enzymes. The amylase was mixed with starch at temperatures of 0℃‚ 37℃‚ 57℃‚ and 90℃. Iodine was added to each mixture and colour changes in each case. Bacteria amylase was found to be effective at 55 0C as the temperature dropped drastically from 4.58℃ to 2.33℃. This shows that the amylase catabolized a lot of starch hence little is left which cannot turn the iodine solution to blue-black which indicates the presence of starch. Fungal amylase is denatured at 90 ℃. This was
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Results Table 1: Experimental results for part A Trial 1(L) Trial 2(L) Trial 3(L) Trial 4(L) Trial 5(L) Initial Reading 0 0 0 0.4 0.9 Final Reading 19.7 19.1 18.9 19.7 19.9 Titre Value 19.7 19.1 18.9 19.3 19 Table 1 above displays the titres obtained during 5 trials in part A of this assay. The titre values procured in trial 2‚ trial 3 and trial 5 manifests an agreement within 0.1mL. However‚ the result achieved in trial 1 and 4 are outliers because of their distance from other observations
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microscopic examination‚ starch grains‚ onion cells and cheek cells are observed .Difference between starch grains‚ onion cells and cheek cells could be differentiate. Materials: * * Small piece of potato‚ * distilled water‚ * iodine solution * a petal of onion * methylene blue solution * cheek cells. Apparatus: * * Cork barrier * glass slides * cover slips * light microscope * Forceps * Knife * filter paper * Dropper
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out of 4 given samples A‚ B‚ C and D. Research Question: Out of the four given samples which sample consists of protein/ starch/reducing sugar/ disaccharide? What color changes will help to identify the contents of each sample when treated with iodine‚ biuret agent and benedicts solution? Theory and background information: What is a protein?1 Proteins are macromolecules‚ consisting of one or more chains of amino acid residues. Proteins perform a vast array of functions within living organisms
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thiosulphate versus time from the start of the reaction was plotted from which the order of reaction with respect to iodine was deduced. Method 2. Determining the rate of a reaction with respect to iodine Using the burettes the four mixtures of hydrochloric acid (HCl)‚ propanone (CH3COCH3) solution and water were made up in the conical flasks according to the table 2. The volumes of iodine needed for each run were measured out from the burette into four test tubes. The tubes were marked as w‚ x‚ y and
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(2013‚ February 21). Amylase. Retrieved February 27‚ 2013‚ from Wikipedia: http://en.wikipedia.org/wiki/Amylase Introduction: Aim: is to find out what the pH level of Amylase is. Variables: Controlled variables: are amount of amylase‚ starch‚ iodine‚ buffer solution and time. Independent variables: are the different buffer solutions being used. Dependant variables: are the different pH levels being registered. Hypothesis: My hypothesis is that the Amylase will register at around a
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positive metal ions in the solutions are less powerful reducing agents‚ then the metal in the plate will be reduced and hence form a deposition of solid metal. e.g. Zn + Pb2+-> Zn2+ + Pb EXPERIMENT 2: Aim: To range the halogens bromine‚ chlorine and iodine‚ with the strongest oxidising agent first. Hypothesis: A strong oxidising agent is a species that easily gains electrons‚ and is hence easily reduced. The ionisation energy of the halogens (Cl2‚ I2‚ Br2) decreases down the group‚ which means that
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Biuret Test for amino groups present in proteins. The Iodine Test to identify the presence of starch. The Grease Spot Test and the Sudan Dye test to identify the presence of lipids (fatty acids). The Benedict’s Test to identify the presence of reducing sugars. In the biuret test if the test result for protein changes a color of lavender‚ purple or pink‚ the test result for protein is positive using the Biuret reagent. In the iodine test If the Iodine turns black or blue-black there is starch present
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focused on finding the ratio of reactants to products to be either 1 to 1 or 1 to 2. In our case the reactants was Lead (II) Nitrate and Potassium Iodine. These two when mixed together make Lead Iodide and Potassium Nitrate. We also had to try and find if the number of moles of Lead(II) Nitrate was the same as the final number of moles for Lead Iodine after the experiment. Our data for the lab had pinpoint accuracy. Proved by the data table below Trials Volume of Pb(NO3)2 Mol Pb(NO3)2 Mol KI Mass
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study the structure of cells under different magnifications. To differentiate between the structure of plant cells and animal cells. To learn the steps and techniques to use a microscope. Materials: Potato‚ onion‚ cheek cell‚ distilled water‚ iodine solution‚ filter paper‚ toothpick and methylene blue solution. Apparatus: Knife‚ light microscope‚ glass slide‚ cover slip‚ forceps‚ mounting needle‚ dropper and white tile.Methods: I) Observation of starch grains The potato was cut to obtain
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