Biology Computational Systems Biology Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations Reactions of two substrates
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F7 Essay Writing (Kinetics) Q. Write an essay on factors which affect the rate of reactions and discuss the uses of kinetic studies. Outlines: (I) Factors affecting the Rate of Reaction (a) temperature ---- collision of molecules with different velocities and kinetic energies ---- Collision Theory and Maxwell-Boltzmann distribution ---- Arrhenius equation and Activation energy (b) concentration ---- frequency of collisions and effective collisions
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WHAT IS TORQUE? Torque is a measure of how much a force acting on an object causes that object to rotate. The object rotates about an axis‚ which we will call the pivot point‚ and will label ’O ’. We will call the force ’F ’. The distance from the pivot point to the point where the force acts is called the moment arm‚ and is denoted by ’r ’. Note that this distance‚ ’r ’‚ is also a vector‚ and points from the axis of rotation to the point where the force acts. (Refer to Figure 1 for a pictoral
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2.6 Kinetic studies of prepared complexes The integral method of Coats–Redfern equation[19‚21‚27‚38] was used for determining the kinetic parameters of the decompositions process for the investigated metal complexes according to following equation: log[log(w_∞/(w_∞-w))⁄T^2 ]〖=log[AR/〖∅E〗^* (1-2RT⁄E^≠ )]〗-E^≠/2.303R 1/T (4) Where w_∞ is the mass loss at the accomplishment of the decomposition reaction‚ w is the mass loss at temperature T‚ ∅ is the rate of heating and R is
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Discussion Our experiment is divided into 9 parts: A. Effect of Nature of Reactants to the reaction rate. B. Effect of Temperature to the reaction C. Effect of Concentration to the Reaction Rate D. Effect of Catalyst to the Reaction Rate E. Chromate-Dichromate Equilibrium F. Thiocyanatoiron (III) Complex Ion Equilibrium G. Weak Acid Equilibrium (Ionization of Acetic Acid) H. Weak Base Equilibrium Ionization of Ammonia I. Saturated Salt (Sodium Chloride) Equilibrium On part (A) we are
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Biochemistry Unit The Kinetics of Alkali Phosphatase Inhibition 1. OVERVIEW This practical builds on the enzymology lab skills you learned in the Acid Phosphatase practical. Again‚ you will measure the initial reaction velocity (V 0) of an enzyme reaction‚ but this time in the absence and then presence of an inhibitor. Last time you used Acid Phosphatase (Prac 1)‚ but this time you will use the enzyme Alkali Phosphatase. These enzymes have different primary (and hence tertiary) structures
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Lecture No. 1 Chemical Kinetics 1.1 The Rate of a Reaction Chemical Kinetics is the area of Chemistry that is concerned with the speed‚ rate or mechanism at which a chemical reaction occurs. Reaction Rate is the change in the concentration of a reactant or product with time (i.e. M/s). It measures how fast a reactant is consumed and how fast a product is formed. 1.2 WRITING RATE EXPRESSIONS Consider the following hypothetical reaction. A + 2B ( 3C + D Rate = - rate
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Centre Course Year/ Trimester Session : Centre for Foundation Studies (CFS) : Foundation in Science : Year 1 / Trimester 1 : 201401 Unit Code Unit Title : FHSC1014 : Mechanics Tutorial 4: Application of Newton’s Laws. 1. The distance between two telephone poles is 50.0 m. When a 1.00 kg bird lands on
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The two different types of energy are kinetic and potential energy. Kinetic energy is the energy a moving object has because of its motion. The kinetic energy of a moving object depends on the object’s mass and its speed. The kinetic energy of a moving object can be calculated from this equation: Kinetic energy (in joules) = ½ mass (in kg) x [speed (in m/s)]² KE= ½ mv² In this equation ^‚ the symbol v represents speed. Example Find the kinetic energy of the ball having mass 0‚5 kg and
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Kinetic Theory - Worksheet 1. State three (3) assumptions of the kinetic theory as it relates to gases. [3] ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ 2. The kinetic theory assumes that all gases are ideal‚ however‚ this does not exist in reality. a. State the conditions under which gases deviate from ideal behaviour and explain
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