Arielle Fisher GFP Lab November 16‚ 2011 Figure 1 A. B. C. D. Figure 1. Confocal images at 400x magnification of HeLa cells to locate GFP activity. HeLa cells were (A) tagged with Green Fluorescent Protein (GFP) (B) labeled with GFP and 14.13 µl Dexamethasone (C) tagged with GFP fused to the glucocorticoid binding
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ions Reagent/ Condition; Reaction | Explanation | Oxygen supply; Combustion | Limited supply of oxygen: CO formed.Even less Oxygen: C is deposited as soot. Excess Oxygen: Complete combustion (giving CO2 and H2O) | Al2 O3 and vaporisation of alkane at 500°C; Cracking of Alkanes | Al2 O3 is used as a catalyst. Heat provides energy for breakage of C-C bonds. | UV light; Initiation step of FRS of alkanes by halogens * Not required for electrophilic addition reaction btw Halogens and alkenes
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original state and the percent of the hydrate recovered was calculated by using the mass of the rehydrated sample by the mass of the original hydrate and then multiplied by 100%. Data Presentation & Analysis Table 1: The data was collected from the lab experiment. Sample calculations are shown. Mass of beaker with sample 30.765g Mass of empty beaker 30.263g Mass of sample .502g Mass of beaker with sample after 1st heat 30.661g Mass of beaker with sample after 2nd heat 30.657g Heating mass
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FACULTY OF ENGINBERING AND BUILT ENVIRONMENT BEng (Hons) Civil Engineering Structure I Deflection Contents: Introduction 3 Objectives 3 Apparatus 4 Procedure 4 Results 4 Discussion 7 Conclusion 7 References 8 Introduction: The deflections of a beam are an engineering concern as they can create an unstable structure if they are large. People don’t want to work in a building in which the floor beams deflect an excessive amount
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the rapid pH change around the equivalence point‚ the titrant has to be added in lesser and lesser amounts as we approach the equivalence point. Procedure: CHM 113 Lab Manual‚ 2014‚ Determination of : Titration of a Weak Acid‚ pgs.89-96 Equations/Experimental Equipment and Apparatus: LoggerPro‚ LabPro‚ pH probe‚ drop counter‚ 60 mL reagent reservoir‚ stir station =p Data and Observations: Data: Original pH=3.23
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11:30-2:20 Abstract: In this experiment‚ phenylmagnesium bromide‚ a Grignard reagent was synthesized from bromobenzene and magnesium strips in a diethyl ether solvent. The Grignard reagent was then converted to triphenylmethanol‚ a tertiary alcohol with HCl. The reaction for phenylmagnesium bromide was: The reaction for Grignard to triphenylmethanol was: In the formation of the Grignard reagent‚ the limited reagent‚ magnesium was determined and 0.00617mol was calculated. In the second part
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Effects of Electrical Stimuli and Injected Reagents on Frog Hearts Melissa Higdon Section 05‚ Group 01 November 19‚ 2013 Introduction: The heart is a very complex muscle for all species. It is responsible for sending oxygenated blood throughout the body as well as sending deoxygenated blood to the lungs‚ and continuously circulate this way for as long as we are alive. Many things can be effected‚ for example how fast the heart beats or how much
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6 November 2012 SUBJECT: Lab Report Analysis This memo proposes the observations and claims I collected from reviewing three different lab reports. Three fields of study are composed within this memo that includes Electrical Engineering‚ Environmental Engineering‚ and Petroleum Engineering. “Electrical Filters‚” (Electrical)‚ written by Joe Schmoe‚ is a lab report made by a student at a university. The College Board produced an environmental lab report named‚ “Monitoring Air Quality‚”
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The Folin assay overestimated the chymotrypsin concentration because of the difference in the properties of BSA and chymotrypsin. The different amino acid composition could have had an effect on how much the Folin reagent was reduced which causes the observed colour change. Too many of these amino acids which have this reducing ability in chymotrypsin could have led to the higher observed concentration seen in this
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Aspirin Synthesis Introduction Out of all the pharmaceutical drugs in the worlds‚ aspirin is made the most. Any potential pharmacist must be familiar with synthesizing the drug. The goal of this lab is to synthesize as much pure aspirin as possible. The reactants‚ acetyl anhydride and salicylic acid‚ must react in phosphoric acid. With phosphoric acid as a catalyst‚ the reaction yields aspirin and acetic acid. The equation for the reaction is as follows: “(CH3CO)2O + HOC6H4COOH □(→┴yields ) CH3CO2C6H4CO2H
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